|
Chiral column screening, method optimization, and small-scale
enantiomeric purification services are available from Supelco/Sigma-Aldrich.
Please contact Supelco/Sigma-Aldrich Technical
Service (Phone: 814-359-3041) to discuss your chiral application request;
you may have to fill out the sample submission form (PDF
version, Word version) ahead of time.
HPLC Chiral column screening
HPLC chiral column screening protocol includes 3 mobile phase conditions run
using 6 different chiral stationary phases. Positive separation is verified
on a separate system. Enantiomers are identified as (+) and (-) using the Chiralyser
optical rotation detection system.
GC Chiral column screening
GC column screening involves exploration of 4 GC chiral phases. Samples that
require derivatization are verified by GC-MS.
HPLC and GC Chiral method optimization
To establish the most efficient method, an optimization study can be conducted
as an outcome of the initial column screening. Method optimization may vary,
depending on the intended use of the method. Some typical uses include:
· Isolation/purification of enantiomers
· Resolution of metabolites
· Establishment of minimum detection limits
· LC-MS compatible methods for clinical, stability, or dissolution studies
Typical experiments in the optimization study include modifying buffer and
pH, organic modifier type and strength, and column temperature.
Small-scale enantiomeric purification
When isolation of enantiomers is required, purification is required. The output
of a small-scale purification project can be milligram to gram quantities, depending
on the needs of the client. Typical enantiomeric purity is 98% and verification
is determined by analytical methods established in the screening study.
Pricing, Turnaround time, and Deliverables
HPLC and GC chiral column screening is typically completed within 3 working
days. A full report is supplied and a column is recommended upon completion
of the project. A charge for the service is incurred whether or not a separation
is identified. At that time, optimization studies can be started as desired
by the client. Standard pricing has been set up for chiral screening. Optimization
and purification are quoted on a project-by-project basis. Contact Technical
Service to discuss your requirements.
|
