- Ordering Information
- Features
Separates α-hydroxy carboxylic acids, amino acids and other α-bifunctional
compounds
High selectivity with simple mobile phases
Copper complex read @ 254nm UV
Simple reversal of elution order, CLC-L vs. CLC-D
Excellent reproducibility
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The Astec CLC phases are based on coupling an enantiomeric form of an amine
to a proprietary Astec derivative to create an appropriate distance for copper
coupling. Using the copper ligand concept, this new phase resolves hydroxy acids
like lactic, malic, tartaric and mandelic. This phase can also resolve amino
acids and other amines by the same mechanism. It has been reported that, in
addition to amino acids, other bifunctional racemates like amino alcohols can
be resolved. In theory, any analyte that can complete the coordination with
the copper ion can be resolved. For the CLC-D column,the L enantiomer generally
elutes before D with the exception of tartaric acid where the D elutes first.
The CLC-L column has the opposite elution order and the D enantiomer elutes
before L.
Generally, we recommend the CHIROBIOTIC T for all amino acid separations that
are detected at 200-210nm UV. Two amino acids in particular have worked better
on the Astec CLC column for low levels of detection since the copper complex
is detected at 254nm UV. They are proline and aspartic acid. Both of these amino
acids can be resolved on the CLC-D or CLC-L in 5mM CuSO4 with the usual reversal
of elution order from the CLC-D to CLC-L. Other analytes separated include free
α-amino acids, malic acid and 3-phenyl lactate.
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