| Anion-Exchange
Columns |
back to top |
| Description |
Cat. No. |
| Column |
|
| DEAE-5PW particle size 10 µm length ×
I.D. 7.5 cm × 7.5 mm |
807164 |
| DEAE-2SW particle size 5 µm length ×
I.D. 25 cm × 4.6 mm |
807168 |
| DEAE-NPR particle size 2.5 µm length ×
I.D. 3.5 cm × 4.6 mm |
813075 |
| Guard Column Kit1 |
|
| DEAE-NPW particle size 5 µm length ×
I.D. 0.5 cm × 4.6 mm |
817088 |
| DEAE-5PW particle size 20 µm length ×
I.D. 1 cm × 6.0 mm |
807210 |
| DEAE-SW particle size 10 µm length ×
I.D. 1 cm × 6.0 mm |
807648 |
|
| Cation-Exchange Columns |
back to top |
| Description |
Cat. No. |
| Column |
|
| CM-5PW particle size 10 µm length ×
I.D. 7.5 cm × 7.5 mm |
813068 |
| SP-NPR particle size 2.5 µm length ×
I.D. 3.5 cm × 4.6 mm |
813076 |
| SP-5PW particle size 10 µm length ×
I.D. 7.5 cm × 7.5 mm |
807161 |
| CM-2SW particle size 5 µm length ×
I.D. 25 cm × 4.6 mm |
807167 |
| CM-3SW particle size 10 µm length ×
I.D. 7.5 cm × 7.5 mm |
807162 |
| Guard Column Kit1 |
|
| CM-5PW particle size 20 µm length ×
I.D. 1 cm × 6 mm |
813069 |
| SP-5PW particle size 20 µm length ×
I.D. 1 cm × 6 mm |
807211 |
| CM-SW particle size 10 µm length ×
I.D. 1 cm × 6 mm |
807650 |
| 1 Guard column kit contains one cartridge, a stand-alone
holder, 5 mL packing, 5 cm of 1/16 in. tubing, and 2 nuts and ferrules. |
|
| |
|
| Additional resources |
|
| Title |
| Ion
Exchange Columns (45 Kb PDF) id. T494077 |
| TSK-GEL
NPR Columns (87 Kb PDF) id. T109862 |
 | This presentation requires the Acrobat plug-in for proper viewing. Download the reader for FREE. | |
|
| Size
Exclusion |
|
| TSK-GEL SW Series Gel Filtration
Columns (Silica-Based) |
back to top |
TSK-GEL SW and TSK-GEL SWXL
columns contain silica-based, hydrophilic bonded phase packings that minimize
interaction with proteins. A 30 cm TSK-GEL SWXL column and a
60 cm TSK-GEL SW column provide similar resolution, but the SWXL
column requires half the analysis time. Sample capacity increases in proportion
with column length.
Because TSK-GEL SWXL and TSK-GEL SW columns are silica based,
they must be operated within the recommended pH range of 2.5 - 7.5. Detailed
operating conditions are described in the information accompanying the columns.
We recommend protecting these columns with the appropriate SWXL
or SW guard column. |
| Glutathione S-Transferase |
| Column: |
TSK-GEL G3000SWXL, 30 cm x 7.8 mm I.D., 5 µm particles |
| Cat. No.: |
808541 |
| Mobile Phase: |
0.3M NaCl/0.05M NaH2PO4, pH
7.0 |
| Flow Rate: |
1 mL/min |
| Temp.: |
25 °C |
| Det.: |
UV, 220 nm |
| Injection: |
100 µL crude glutathione S-transferase preparation
from guinea pig liver (0.7mg) |
| |
 |
|
| Description |
Cat. No. |
| G2000SWXL particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
808540 |
| G3000SWXL particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
808541 |
| G4000SWXL particle size 8 µm length
× I.D. 30 cm × 7.8 mm |
808542 |
| Super SW2000 particle size 4 µm length
× I.D. 30 cm × 4.6 mm |
818674 |
| Super SW3000 particle size 4 µm length
× I.D. 30 cm × 4.6 mm |
818675 |
| G2000SW particle size 10 µm length ×
I.D. 30 cm × 7.5 mm |
805788 |
| G2000SW particle size 10 µm length ×
I.D. 60 cm × 7.5 mm |
805102 |
| G3000SW particle size 10 µm length ×
I.D. 30 cm × 7.5 mm |
805789 |
| G3000SW particle size 10 µm length ×
I.D. 60 cm × 7.5 mm |
805103 |
| G3000SW particle size 13 µm length ×
I.D. 60 cm × 21.5 mm |
805147 |
| G4000SW particle size 13 µm length ×
I.D. 30 cm × 7.5 mm |
805790 |
| G4000SW particle size 13 µm length ×
I.D. 60 cm × 7.5 mm |
805104 |
TSK-GEL
Column |
Particle Size
(µm) |
Pore Size (Å) |
Sample MW
(globular proteins) |
| Super SW2000 |
4 |
125 |
5-150 x 103 |
| G2000SWXL |
5 |
125 |
5-150 x 103 |
| G2000SW |
10 |
125 |
5-100 x 103 |
| Super SW3000 |
4 |
250 |
10-500 x 103 |
| G3000SWXL |
5 |
250 |
10-500 x 103 |
| G3000SW |
10 |
250 |
10-500 x 103 |
| G4000SWXL |
8 |
450 |
20-10,000 x 103 |
| G4000SW |
13 |
450 |
20-10,000 x 103 |
| Mobile Phase: 0.03 M NaCl in 0.1 M
phosphate buffer, pH 7.0 |
|
|
| Additional resources |
|
| Title |
| TSK-GEL
SW and SWXL Columns (56 Kb PDF) id. T494076 |
| |
| TSK-GEL
PW Series Gel Filtration Columns (Polymer-Based) |
back to top |
TSK-GEL PW and TSK-GEL PWXL
columns are used in high performance gel filtration separations of water-soluble
polymers and oligosaccharides. The hydrophilic polymer matrix has excellent
chemical and mechanical stability. Although commonly used with aqueous solvents,
the polymer is compatible with up to 50% organic solvent.
We recommend using a TSK-GEL PW guard columns with G2500PW - G6000PW columns.
Use a TSK-GEL PWXL guard column with any PWXL column.
Bulk packing can be ordered to repack PW columns and guard columns. |
| Description |
Cat. No. |
| G2500PWXL particle size 6 µm length
× I.D. 30 cm × 7.8 mm |
808020 |
| G3000PWXL particle size 6 µm length
× I.D. 30 cm × 7.8 mm |
808021 |
| G4000PWXL particle size 10 µm length
× I.D. 30 cm × 7.8 mm |
808022 |
| G5000PWXL particle size 10 µm length
× I.D. 30 cm × 7.8 mm |
808023 |
| G6000PWXL particle size 13 µm length
× I.D. 30 cm × 7.8 mm |
808024 |
| G2000PW particle size 10 µm length ×
I.D. 30 cm × 7.5 mm |
805761 |
| G2500PW particle size 10 µm length ×
I.D. 30 cm × 7.5 mm |
808028 |
| G3000PW particle size 10 µm length ×
I.D. 30 cm × 7.5 mm |
805762 |
| G4000PW particle size 17 µm length ×
I.D. 30 cm × 7.5 mm |
805763 |
| G5000PW particle size 17 µm length ×
I.D. 30 cm × 7.5 mm |
805764 |
| G6000PW particle size 17 µm length ×
I.D. 30 cm × 7.5 mm |
805765 |
TSK-GEL
Column |
Particle Size
(µm) |
Pore Size (Å) |
Sample MW
(PEGS/PEOs) |
Sample MW
Dextrans |
| G-Oligo-PW |
6 |
125 |
<2,000 |
- |
| G2000PW |
10 |
125 |
<2,000 |
- |
| G2500PWXL |
6 |
<200 |
<3,000 |
- |
| G2500PW |
10 |
<200 |
<3,000 |
- |
| G3000PWXL |
6 |
200 |
<50,000 |
<60,000 |
| G3000PW |
10 |
200 |
<50,000 |
<60,000 |
| G4000PWXL |
10 |
500 |
2,000-300,000 |
1,000-700,000 |
| G4000PW |
17 |
500 |
2,000-300,000 |
1,000-700,000 |
| G5000PWXL |
10 |
1,000 |
4,000-1,000,000 |
50,000-7,000,000 |
| G5000PW |
17 |
100 |
4,000-1,000,000 |
4,000-1,000,000 |
| G6000PWXL |
13 |
>1,000 |
40,000-8,000,000 |
500,000- 50,000,000 |
| G6000PW |
17 |
>1,000 |
40,000-8,000,000 |
500,000-50,000,000 |
| G-DNA-PW |
10 |
4,000 |
40,000-8,000,000 |
- |
| GMPWXL |
13 |
<100-1000 |
500-8,000,000 |
<50,000,000 |
| GMPW |
17 |
<100-1000 |
500-8,000,000 |
<50,000,000 |
Mobile Phase: Polyethylene glycols/polyethylene
oxides—distilled water
Mobile Phase: Dextrans—0.2 M phosphate buffer, pH 6.8 |
|
|
| Additional resources |
|
| Title |
| TSK-GEL
PW and PWXL Columns (71 Kb PDF) id. T494075 |
| |
| TSK-GEL
HHR Series Gel Permeation Columns |
back to top |
| TSK-GEL H series gel permeation columns are
stable in solvents having a wide range of polarities. The particles do not
swell or shrink as the solvent is changed from toluene through methanol.
However, these columns cannot be used with polar solvents, such as water
or water:methanol mixtures. Spherical 5 µm polystyrene/divinylbenzene
particles provide a minimum of 16,000 plates per 30 cm x 7.8 mm I.D. column.
Eight pore sizes are available, ranging from an exclusion limit of about
2,000 Daltons for G1000HHR columns to more than 10,000,000 Daltons
for G7000HHR columns. The four mixed bed columns (H, L, M, N)
feature extended linear molecular weight operating ranges for sample screening
or more formal analyses. |
| (see table after HXL columns
for Analyte Molecular Weight Range for these columns) |
| Description |
Cat. No. |
| G1000HHR particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817352 |
| G2000HHR particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817353 |
| G2500HHR particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817354 |
| G3000HHR particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817355 |
| G4000HHR particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817356 |
| G5000HHR particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817357 |
| GMHHR-L particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817362 |
| GMHHR-M particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
817392 |
| Guard Column |
|
| HHR-L Guard column particle size 7 µm length
× I.D. 4.0 cm × 6.0 mm1 |
817368 |
| 1 Use to protect G1000HHR
- G4000HHR and GMHHR-L columns. |
| |
| Additional resources |
|
| Title |
| TSK-GEL
HHR Columns (68 Kb PDF) id. T496085 |
| | This presentation requires the Acrobat plug-in for proper viewing. Download the reader for FREE. | |
| |
| TSK-GEL
HXL Series Gel Permeation Columns |
back to top |
| The TSK-GEL HHR and TSK-GEL HXL
columns perform very similarly. The HXL columns were developed
as an upgrade from the previous series, the TSK-GEL H. The HXL
can be converted to different mobile phases for doing non-aqueous polymer
separations, but it was found that the performance deteriorated when the
conversion occurred. The HHR columns were developed to allow easy conversion
between different mobile phases. The HHR is considered exceptionally
stable, and is easily converted to other mobile phases with little loss
in theoretical plates. The HXL columns are a good choice if your
mobile phase of choice is THF and you do not plan on changing mobile phases.
If you will change mobile phases or your mobile phase of choice is not THF,
the HHR columns should be used. |
| Description |
Cat. No. |
| G1000HXL particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
816131 |
| G2000HXL particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
816134 |
| G2500HXL particle size 5 µm length
× I.D. 30 cm × 7.8 mm |
816135 |
| G3000HXL particle size 6 µm length
× I.D. 30 cm × 7.8 mm |
816136 |
| G4000HXL particle size 6 µm length
× I.D. 30 cm × 7.8 mm |
816137 |
| G5000HXL particle size 9 µm
length × I.D. 30 cm × 7.8 mm |
816138 |
| G6000HXL particle size 9 µm length
× I.D. 30 cm × 7.8 mm |
816139 |
| GMHXL particle size 9 µm length
× I.D. 30 cm × 7.8 mm |
816141 |
| GMHXL-HT particle size 13 µm length
× I.D. 30 cm × 7.8 mm |
807112 |
| Guard Column |
|
| HXL-L Guard column particle size 6 µm length
× I.D. 4.0 cm × 6.0 mm1 |
807113 |
| HXL-H Guard column particle size 13 µm length
× I.D. 4.0 cm × 6.0 mm2 |
813727 |
1 Use to protect
G1000HXL - G4000HXL columns.
2 Use
to protect G5000HXL - G6000HXK columns. |
| Column |
Analyte Molecular
Weight Range (Daltons) |
| G1000H |
<1,500 |
| G2000H |
<4,000 |
| G2500H |
<1.2 x 104 |
| G3000H |
<3.0 x 104 |
| G4000H |
<5.5 x 105 |
| G5000H |
<1.5 x 106 |
| G6000H |
<~1 x 107 |
| G7000H |
<~5 x 107 |
| GMH-H |
<~1 x 107 |
| GMH-L |
<1.0 x 104 |
| GMH-M |
<1.0 x 106 |
|
|
|
| TSK-GEL Reversed-Phase
Columns |
back to top |
| |
| Description |
Cat. No. |
| Silica-Based Column |
|
| Oligo-DNA RP particle size 5 µm length
× I.D. 15 cm × 4.6 mm |
813352 |
| Super ODS particle size 2 µm length ×
I.D. 5 cm × 4.6 mm |
818154 |
| Super ODS particle size 2 µm length ×
I.D. 10 cm × 4.6 mm |
818197 |
| Resin-Based Column |
|
| C18-NPR particle size 2.5 µm length ×
I.D. 3.5 cm × 4.6 mm |
814005 |
| C18-4PW particle size 7 µm length ×
I.D. 15 cm × 4.6 mm |
813351 |
| Phenyl-5PW RP particle size 10 µm length
× I.D. 7.5 cm × 4.6 mm |
808043 |
| Guard Filters |
|
| Guard Filter |
818207 |
| Stand-Alone Holder for Filter |
818206 |
|
| TSK-GEL Affinity Columns |
back to top |
| High efficiency, resin-based TSK-GEL affinity
columns (10 µm particles) separate or purify many enzymes and other
proteins. The table below describes the characteristics of the columns offered. |
| |
Characteristics of TSK-GEL Affinity Columns
Immobilized
Ligand |
Adsorption Capacity
(per mL Gel) |
Typical Uses |
| ABA-5PW |
|
|
| p-amino benzamidine |
3-4 mg trypsin |
trypsin, bovine thrombin,
urokinase, enterokinase,
plasminogen activator |
| Boronate-5PW |
|
|
| m-aminophenyl boronic acid |
40 µmol sorbitol |
glycoproteins, nucleases,
nucleotides, catecholamines,
carbohydrates, transfer RNAs |
| Chelate-5PW |
|
|
| iminodiacetic acid |
~20 µmol
Cu+2 or Zn+2 |
serum proteins, interferon,
collagenase, granule protein,
plasminogen activator, lactoferrin |
|
|
| Description |
Cat. No. |
| ABA-5PW particle size 10 µm length ×
I.D. 7.5 cm × 7.5 mm |
813067 |
| Boronate-5PW particle size 10 µm length
× I.D. 7.5 cm × 7.5 mm |
813066 |
| Chelate-5PW particle size 10 µm length
× I.D. 7.5 cm × 7.5 mm |
808645 |
| Guard Column Kit1 |
|
| Chelate-5PW particle size 20 µm length
× I.D. 1 cm × 6.0 mm |
808647 |
| 1 Guard column kit contains one cartridge,
a stand-alone holder, 5 mL packing, 5 cm of 1/16 in. tubing, and 2 nuts
and ferrules. |
|
| TSK-GEL Hydrophobic Interaction
Columns |
back to top |
Both hydrophobic interaction chromatography
(HIC) and reversed-phase liquid chromatography separate on the basis of
protein hydrophobicity and allow selective binding and desorption of proteins.
However, HIC operates at significantly lower binding energy and uses aqueous
mobile phases. These characteristics provide a gentle technique that is
less likely to disturb protein conformation. As a result, HIC generally
provides better activity recovery.
TSK-GEL Ether-5PW, Phenyl-5PW, and Butyl-NPR resin-based columns provide
a range of hydrophobicities for chromatographic optimization. Ammonium sulfate
concentration can be minimized by using a very hydrophobic packing (e.g.,butyl).
We recommend an Ether-5PW column for purifying very hydrophobic proteins.
Ether-5PW and Phenyl- 5PW packings are based on TSK-GEL G5000PW resin -
10 µm particles with 1000 Å pores. Butyl-NPR packing is prepared
from 2.5 µm nonporous particles, allowing rapid analyses. All three
column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by
using corresponding Toyopearl bulk resins. |
| Proteins, mixture |
| Column: |
TSK-GEL Phenyl-5PW, 7.5 cm × 7.5 mm I.D., 10 µm
particles |
| Cat. No.: |
807573 |
| Mobile Phase: |
0.1 M phosphate buffer, pH 7.0 plus (NH4)2SO4,
1.8 M to 0 M in 60 min |
| Flow Rate: |
1 mL/min |
| Det.: |
UV, 280 nm |
| |
 |
|
| Description |
Cat. No. |
| Butyl-5PW particle size 2.5 µm length ×
I.D. 3.5 cm × 4.6 mm |
814947 |
| Ether-5PW particle size 10 µm length ×
I.D. 7.5 cm × 7.5 mm |
808641 |
| Phenyl-5PW particle size 10 µm length ×
I.D. 7.5 cm × 7.5 mm |
807573 |
| Guard Column Kit1 |
|
| Phenyl-5PW particle size 20 µm length ×
I.D. 1 cm × 6.0 mm |
807652 |
| 1 Guard column kit contains one cartridge,
a stand-alone holder, 5 mL packing, 5 cm of 1/16 in. tubing, and 2 nuts
and ferrules. |
