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The threat of bioterrorism, war and natural outbreaks require the exhaustive of control for insidious organisms. Bacillus anthracis is one of the
most notorious controlled species.
Fluka is now introducing a medium, recommended by the WHO for determining Bacillus anthracis (Fluka Cat. No. 55678). This medium is called PLET Agar after the initials of Polymyxin B - Lysozym - EDTA and Thallous acetate. In order to make it selective, an Anthracis Selective Supplement (Fluka Cat. No. 72659) must be added. Organisms such as Bacillus cereus are inhibited. Bacillus anthracis appears after 36-40 hours incubation at 37°C as a circular, creamy-white colony with a ground glass texture.
References
| (1) |
R.F. Knisley J. Bacteriol., 92, 784 (1966) |
| (2) |
J.R. Norris, C.W. Berkley, N.A. Logan, A.G. O’Donnell,
In M.P. Starr et al (ed), The Prokaryotes: a Handbook on Habitats, Isolation
and Identification of Bacteria, vol. 2, Springer Verlag, Berlin (1981) |
| (3) |
J.M. Parry P.C.B. Turnbull, J.R. Gibson, A Colour Atlas of
Bacillus species, Wolfe Medical Publications, London, UK (1983) |
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