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Microbiology


Use and Selection of Culture Media


Convenient dehydrated culture media of the highest quality are supplied for the growth of a wide range of heterotrophic organisms. For organisms with more diverse nutritional requirements, Fluka offers a large selection of media ingredients ranging from simple sugars to pH and redox indicators. A selection of commonly used dyes and additives is also included.

Other components, e.g. water soluble salts of trace elements, vitamins, antibiotics amino acids, purines, pyrimidines, chemical agents to induce mutation and inhibitors, etc., can be found in the Fluka catalogue on Chemika-Biochemica.

The Dehydrated Culture Media range is continuously being expanded. Please contact us for further information.


Environmental Analysis

In the field of environmental microbiology most cultures have to be isolated and enriched from their natural habitats. With this in mind, Fluka supplies a range of media additives necessary for specialized media. For the detection and enumeration of microorganisms in complex natural systems such as soil, Fluka offers a range of fluorescent dyes.


Soil Analysis


11703 Bacillus Medium 70148 Nutrient Agar
70133 Blood Agar (Base) 70116 Nutrient Agar No 2
22310 Cereus Selective Agar 70122 Nutrient Broth No 1
27548 Clostridium Agar 70123 Nutrient Broth No 2
60865 Cooked Meat Broth 70149 Nutrient Broth No 3
70185 Czapek Dox Agar 70151 Nutrient Gelatin
48726 Gelatine Phosphate Buffer 77412 PGYT Broth
60788 King Agar A 82618 PYR Broth
60786 King Agar B 83331 PYS Broth
61348 Lactose Gelatin Broth 88199 TG Broth
14305 Motility Nitrate Agar 93745 TSC Agar
72548 Nitrate Broth 70196 Wort Agar

Water Analysis

00185 Acetamide Nutrient Broth 44657 ECD MUG Agar
70132 Azide Blood Agar (Base) 70137 ENDO Agar (Base)
11539 Azide Glucose Broth 45183 Enterococcus Selective Agar
48300 Bile Esculin Agar 70187 Glucose Broth
70133 Blood Agar (Base) 50875 GSP Agar
16016 BRILA MUG Broth 60641 KF-Streptococcus Agar
16750 Bromocresol Purple Azide Broth 60786 King Agar B
22470 Cetrimide Agar 70142 Lactose Broth
70135 DCLS Agar 61749 Lauryl sulfate Broth
31432 DEV ENDO Agar 70143 Mac Conkey Agar No 1
31433 DEV Gelatin Agar 70144 Mac Conkey Broth
31434 DEV Glucose Broth 63014 Mac Conkey MUG Agar
31435 DEV Glutamate Broth 63163 Malachite Green Broth
31436 DEV Lactose Broth 70148 Nutrient Agar
31437 DEV Lactose Peptone Broth 70116 Nutrient AgarNo2
31401 DEV Lactose Peptone MUG Broth 70152 Plate Count Agar
31438 DEV Nutrient Agar 80961 Plate Count MUG Agar
31439 DEV Nutrient Gelatin 85463 Simmons Citrate Agar
31406 DEV Tryptophan Broth 86348 TCBS Agar
44653 EC Broth 70159 Tryptone Glucose Extract Agar

Food and water contaminated by microorganisms (bacteria and yeasts), viruses and protozoa can cause severe disease in humans, especially in people weakened by illness or during convalescence, malnutrition, etc.

There are two categories of foodborne diseases. First, food poisoning is caused by the presence of microbial toxins in food products, e.g. by Staphylococcus aureus, Clostridium perfringens (both produce enterotoxins which elicit enteric disease such as. diarrhoea) and Clostridium botulinum (botulism the most severe type of food poisoning).

Second, food infection is caused by the growth of microorganisms in the body after eating contaminated food, e.g. by Salmonella spp. (salmomonellosis) and Campylobacter jejuni (high fever, abdominal cramps). Many human pathogens are transmitted by fecally contaminated water, the most important are Salmonella typhi (typhoid fever) and Vibrio cholerae (cholera). Microbiological test procedures for the examination of food and water have been standardized and regulated, but nearly every country has its own regulations, the "Schweizerisches Lebensmittelhandbuch" being a case in point. All the necessary culture media, their additives, dyes and indicators which Fluka offers conform to Swiss regulations.



Drinking Water, Mineral- and
Table Water

95388 Bismuth Sulfide Agar 70152 Plate Count Agar
70134 Brilliant Green Agar (modified) 70153 Selenite Broth (Base)
16025 Brilliant Green Bile Lactose Broth 85463 Simmons Citrate Agar
22470 Cetrimide Agar 85640 SS-Agar
70135 DCLS Agar 88151 Tetrathionate Broth
70137 ENDO Agar (Base) 70156 TetrathionateBroth(Base)
47706 Formate Ricinoleate Broth 44940 Triple Sugar Iron Agar
70143 MacConkey Agar No 1 22092 Tryptic Soy Broth
63163 Malachite Green Broth 70159 TryptoneGlucoseExtractAgar
69150 Methyl Red Voges Proskauer Broth 70194 Tryptone water
70149 Nutrient Broth No 3 51463 Urea Broth
77185 Peptone Water 70188 Violet Red Bile Agar
78052 Phenylalanine Agar

Beer and Wine

10825 APT Agar 70152 Plate Count Agar
30912 DeMan-Rogosa-Sharpe Agar 83410 Raka Ray No3 Broth
70143 Mac Conkey Agar No 1 70196 Wort Agar
69964 MRS Agar

Fruits and Vegetables

11705 Baird Parker Agar 70152 Plate Count Agar
16025 Brilliant Green Bile Lactose Broth 70159 Tryptone Glucose Extract Agar
61749 Lauryl sulfate Broth
11705 Baird Parker Agar 70152 Plate Count Agar
44653 EC Broth 70139 Potato Glucose Agar
61749 Lauryl sulfate Broth 70159 Tryptone Glucose Extract Agar
70145 Malt Extract Agar 70188 Violet Red Bile Agar

Sour Vegetables, Vinegar

10825 APT Agar 69965 Mossel Broth
11539 Azide Glucose Broth 70148 Nutrient Agar
11853 Barnes Agar 70116 Nutrient Agar No 2
16025 Brilliant Green Bile Lactose Broth 70139 Potato Glucose Agar
27544 Clostridial Differential Broth 83920 Rogosa Agar
27546 Clostridial Nutrient Medium 84086 Sabouraud 2% Glucose Agar
61724 Liver Broth 70188 Violet Red Bile Agar
70145 Malt Extract Agar 96005 ZATK Agar

Corn and Corn Products

11539 Azide Glucose Broth 70152 Plate Count Agar
11705 Baird Parker Agar 70139 Potato Glucose Agar
95388 Bismuth sulfite Agar 82618 PYR Broth
53286 Brain Heart Broth 83331 PYS Broth
16025 Brilliant Green Bile Lactose Broth 84922 Selenite Cystine Broth
16026 Brilliant Green Phenol Red Lactose Sucrose Agar 85463 Simmons Citrate Agar
27546 Clostridial Nutrient Medium 85640 SS-Agar
44653 EC Broth 70156 Tetrathionate Broth (Base)
70141 Koser Citrate Broth 88148 Tetrathionate Enrichment Broth
according to Muller-Kauffmann
61348 Lactose Gelatin Broth 44940 Triple Sugar Iron Agar
61749 Lauryl sulfate Broth 93745 TSC Agar
62087 Levine EMB Agar 51463 Urea Broth
62915 Lysine Iron Agar 70188 Violet Red Bile Agar
70143 MacConkey Agar No l 70195 Vogel-Johnson Agar
14305 Motility Nitrate Agar

Sugar

31415 Dextrose Caseinpeptone Agar 70152 Plate Count Agar
61724 Liver Broth 70139 Potato Glucose Agar
70148 Nutrient Agar 84086 Sabouraud 2% Glucose Agar
70116 Nutrient Agar No 2 70196 Wort Agar
75405 Orange-serum Agar

Cocoa, Chocolate and Sweets

11539 Azide Glucose Broth 62895 Lysine Decarboxylase Salt Broth
11705 Baird Parker Agar 62915 Lysine Iron Agar
48300 Bile Esculin Agar 70143 Mac Conkey Agar No 1
95388 Bismuth sulfite Agar 69150 Methyl Red Voges Proskauer Broth
53286 Brain Heart Broth 72548 Nitrate Broth
16025 Brilliant Green Bile Lactose Broth 70151 Nutrient Gelatin
16026 Brilliant Green Phenol Red Lactose
Sucrose Agar
70152 Plate Count Agar
44653 EC Broth 70139 Potato Glucose Agar
51490 Hektoen Enteric Agar 84922 Selenite Cystine Broth
60641 KF-Streptococcus Agar 85640 SS-Agar
60787 Kligler Agar 44940 Triple Sugar Iron Agar
70141 Koser Citrate Broth 70194 Tryptone water
61348 Lactose Gelatin Broth 93745 TSC Agar
61749 Lauryl sulfate Broth 51463 Urea Broth
62087 Levine EMB Agar 70189 Violet Red Bile Glucose Agar
95586 XLD Agar

Spices

11705 Baird Parker Agar 69150 Methyl Red Voges Proskauer Broth
48300 Bile Esculin Agar 14305 Motility Nitrate Agar
95388 Bismuth sulfite Agar 70152 Plate Count Agar
53286 Brain Heart Broth 82618 PYR Broth
16025 Brilliant Green Bile Lactose Broth 83331 PYS Broth
22520 China Blue Lactose Agar 83920 Rogosa Agar
70185 Czapek Dox Agar 85640 SS-Agar
44653 EC Broth 88148 Tetrathionate Enrichment Broth
according to Muller-Kauffmann
60641 KF-Streptococcus Agar 44940 Triple Sugar Iron Agar
70141 Koser Citrate Broth 93745 TSC Agar
61348 Lactose Gelatin Broth 70188 Violet Red Bile Agar
61749 Lauryl suIfate Broth 70195 Vogel-Johnson Agar
62087 Levine EMB Agar 70196 Wort Agar
61724 Liver Broth
62915 Lysine Iron Agar

Meat

10825 APT Agar 72548 Nitrate Broth
11705 Baird Parker Agar 70116 Nutrient Agar No 2
95388 Bismuth sulfite Agar 70151 Nutrient Gelatin
70133 Blood Agar (Base) 75315 OF Test Nutrient Agar
53286 Brain Heart Broth 77187 Peptone Water
phosphate-buffered
27048 Christensen’s Urea Agar 70152 Plate Count Agar
44653 EC Broth 85463 Simmons Citrate Agar
48905 Giolitti Cantoni Broth 85640 SS-Agar
70187 Glucose Broth 88151 Tetrathionate Broth
60641 KF-Streptococcus Agar 44940 Triple Sugar Iron Agar
61348 Lactose Gelatin Broth 70194 Tryptone water
61749 Lauryl suIfate Broth 93745 TSC Agar
62087 Levine EMB Agar 51463 Urea Broth
62895 Lysine Decarboxylase Salt Broth 70189 Violet Red Bile Glucose Agar
62915 Lysine Iron Agar 70195 Vogel-Johnson Agar
70143 Mac Conkey Agar No 1

Fresh and Frozen Fish

10825 APT Agar 70152 Plate Count Agar
70134 Brilliant Green Agar (modified) 83920 Rogosa Agar
16025 Brilliant Green Bile Lactose Broth 85627 SPS Agar
44653 EC Broth 86348 TCBS Agar
49281 Glucose Salt Teepol Broth 91015 Tributyrin Agar
50875 GSP Agar 44940 Triple Sugar Iron Agar
61749 Lauryl sulfate Broth 93745 TSC Agar
62087 Levine EMB Agar 93735 TSN Agar
69965 Mossel Broth 70188 Violet Red Bile Agar
69964 MRS Agar 70195 Vogel-Johnson Agar
75405 Orange-serum Agar 96005 ZATK Agar