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Learn about the use and theory of HPLC separations through three different
seminars. Topics range from fundamentals to selecting the right column. Each
seminar is also broken into segments to allow for more convenient viewing.
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HPLC Column Fundamentals
(28:01) provides an introduction to how solutes can interact with columns
containing porous particles and stationary phases to result in separation.
The efficiency, retention and selectivity terms in the resolution equation
will be explained as well as how certain particle and column characteristics
can alter the terms and affect resolution. |
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The Use of Alternate Selectivity in Reversed-Phase HPLC
(42:52) will describe the chemical structure of various types of bonded
phases. Some possible explanations why polar RP phases have different
selectivity from nonpolar, straight-chain alkyl phases will be proposed,
and classes of compounds that are best separated by polar RP phases will
be shown. |
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Introduction to Ascentis
HPLC Columns (23:04) provides an overview of the family of columns.
Ascentis columns are retentive, high performance phases that are designed
specifically for separation of small molecules. Method development is
made easy because all Ascentis columns have comparable retention. |
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