Certified Fluorescence Standard

For luminescence techniques, there is an increasing need for reliable and comparable data,
quantification, and eventually standardization. This holds especially for applications in strongly
regulated areas like e.g. medical diagnostics.

In close cooperation between Federal Institute for Materials Research and Testing (Berlin, Germany)
and Sigma-Aldrich, the Kit Spectral Fluorescence Standards was developed.

Calibration with our kit rules out instrumentation as major source of variability and improves comparability of fluorescence measurements across instruments and over time. It is a calibration tool for the determination and control of the relative spectral responsivity of fluorescence instruments,
which is

- simple
- flexible
- traceable

The Kit is optimized for spectrofluorometers and can be employed for a broad variety of measurement
geometries. With proper consideration of the underlying measurement principle, it can be adapted to the calibration of other types of fluorescence instruments.


Instrument Effects on Fluorescence Signals
Determination of the Spectral Responsivity
Kit Spectral Fluorescence Standards: Principle and Properties
Tracability, Certification, and Proviniency Testing
References

References

1. J. R. Lakowicz (1999) Principles of Fluorescence Spectroscopy 2nd ed, Kluwer Academic/Plenum Press, New York.
2. J. R. Lakowicz (Ed.) (1992–2004) Topics in Fluorescence Spectroscopy Series Vol. 1–8, Plenum, New York.
3. O. S. Wolfbeis (Series Ed.) (2001–2004) Springer Series on Fluorescence, Methods and Applications Vol. 1–3, Springer, Berlin.
4. S. G. Schulman (Ed.) (1985–1993) Molecular Luminescence Spectroscopy Parts 1–3, Wiley Interscience, New York.
5. U. Resch-Genger, K. Hoffmann, W. Nietfeld, A. Engel, J. Neukammer, R. Nitschke, B. Ebert, and R. Macdonald, (2005), How to improve quality assurance in fluorometry: Fluorescence-inherent sources of error and suited fluorescence standards, J. Fluoresc., 15(3), 337-362 and references therein.
6. U. Resch-Genger, D. Pfeifer, C. Monte, W. Pilz, A. Hoffmann, M. Spieles, K. Rurack, J. Hollandt, D. Taubert, B. Schönenberger, and P. Nording, (2005), Traceability in fluorometry: Part II. Spectral fluorescence standards, J. Fluoresc. 15(3), 315-336.
7. U. Resch-Genger, K. Hoffmann, W. Nietfeld, A. Engel, J. Neukammer, R. Nitschke, B. Ebert, and R. Macdonald, (2005), How to improve quality assurance in fluorometry: Fluorescence-inherent sources of error and suited fluorescence standards, J. Fluoresc., 15(3), 337-362 and references therein.
8. J. W. Eastman, (1966), Standardization of fluorescence spectra and the calibration of spectrofluorimeters, Appl. Opt. 5(7) 1125-1132.
9. K. Hoffmann, C. Monte, D. Pfeifer, and U. Resch-Genger, (2005), Standards in Fluorescence Spectroscopy: Simple Tools for the Characterization of Fluorescence Instruments, G.I.T. Laboratory Journal (5), 2-4.
10. J. N. Miller (1981) Standards in Fluorescence Spectrometry, Ultraviolett Spectrometry Group, London.
11. R. A. Velapoldi and H. H. Tonnesen, (2004), Corrected emission spectra and quantum yields for a series of fluorescent compounds in the visible spectral region, J. Fluoresc. 14(4), 465-472.
12. J. W. Hofstraat, and M. J. Latuhihin, (1994), Correction of fluorescence-spectra, Appl. Spectr. 48(4), 436-447.
13. R. A. Velapoldi and K. D. Mielenz, (1980), A fluorescence standard reference material: Quinine sulfate dihydrate, NBS Spec. Publ. 260-64, PB 80132046, Springfield, VA.
14. J. A. Gardecki and M. Maroncelli, (1998), Set of secondary emission standards for calibration of the spectral responsivity in emission spectroscopy, Appl. Spectr. 52(9), 1179-1189.
15. W. Bremser, P. deRose, D. S. Duewer, B. Ebert, A. Hoffmann, J. Hollandt, R. Macdonald, C. Monte, D. Pfeifer, U. Resch-Genger, D. R. Taubert, J. Zwinkels, manuscript in preparation.

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