| Purified Enzymes |
|
| Product Name |
Source |
Product # |
| Recombinant Firefly |
|
|
| New Luciferase, recombinant, expressed in Escherichia coli, minimum 95% (SDS-PAGE), Buffered aqueous glycerol solution |
Luciola mingrelica |
L4899 |
| New Luciferase, recombinant, expressed in Escherichia coli |
Photinus pyralis |
L1792 |
| Native Firefly |
|
|
Luciferase, Lyophilized powder, 15-30 × 106 light units/mg protein
Reduced Price |
Photinus pyralis |
L9506 |
| Luciferase, Lyophilized powder, minimum 10 × 106 light units/mg protein |
Photinus pyralis |
L1759 |
| Luciferase, Lyophilized powder, 10-25 × 106 light units/mg protein |
Photinus pyralis |
L9009 |
| Bacterial |
|
| Luciferase, Lyophilized powder |
Vibrio fischeri |
L8507 |
| Kits & Reagents for ATP Determination See data below |
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| Product Name |
Source |
Product # |
| Adenosine 5¢-triphosphate (ATP) assay kit |
Photinus pyralis |
FL-AA |
| Adenosine 5¢-triphosphate (ATP) assay mix, Lyophilized powder |
Photinus pyralis |
FL-AAM |
| Adenosine 5¢-triphosphate (ATP) assay mix dilution buffer, Lyophilized powder |
|
FL-AAB |
| Adenosine 5¢-triphosphate (ATP) bioluminescent somatic cell assay kit |
Photinus pyralis |
FL-ASC |
| Adenosine 5¢-triphosphate (ATP) standard disodium salt trihydrate, Lyophilized powder |
|
FL-AAS |
| Somatic cell ATP releasing reagent |
|
FL-SAR |
| Firefly Extracts for Bioluminescence |
|
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| Product Name |
Source |
Product # |
| Firefly Lantern Extract, Powder |
Photinus pyralis |
F3641 |
| Firefly Lantern Extract |
Photinus pyralis |
FLE-250 |
| Firefly diluent, Lyophilized powder |
|
F3766 |
| Substrates |
|
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| Product Name |
Source |
Product # |
| D-Luciferin |
Synthetic |
L9504 |
| D-Luciferin |
Synthetic, SigmaUltra, minimum 99% (HPLC) |
L6152 |
| D-Luciferin sodium salt, Lyophilized powder |
Synthetic |
L6882 |
| Antibodies |
|
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| Product Name |
Specificity |
Product # |
| New Monoclonal Anti-Luciferase from mouse, approx. 2 mg/ml, Buffered aqueous solution, Purified immunoglobulin, Clone LUC-1 |
Photinus pyralis |
L2164 |
| Anti-Luciferase from rabbit, IgG fraction of antiserum, Buffered aqueous solution |
Photinus pyralis |
L0159 |
| | |
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ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible.1,2 When ATP is the limiting reagent, the light emitted is proportional to the ATP present.
