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Sigma® Product # T3568
Recombinant, Animal-Free Bovine Trypsin, Expressed in Corn
Trypzean™ eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
Specifications:
| Appearance: |
White Powder |
| Protein Content: |
³90% [E1% A280 nm = 14.3] |
| Specific Activity: |
Not less than 3650 USP units/mg solid |
| Molecular Weight: |
23,000–27,000 Da by SDS PAGE |
| Solubility: |
500,000 USP Trypsin Units are soluble in 10 mL of water and in 10 mL of saline TS |
Features and Benefits:
- High Purity – Eliminates contaminating activities and increases specificity.
- Free of animal contaminants – Eliminates bovine pathogen concerns.
- Comparable kinetics to native trypsin – Simplifies transition from native source trypsin products.
- Suitability for dissociation of adherent cell lines at several fold lower concentrations than traditional native trypsins – Reduces amount of trypsin required.
- Inhibition by soybean trypsin inhibitor is comparable to native trypsins on a weight to weight basis – Reduces amount of trypsin inhibitor required.
Kinetics of TrypZean™:
Concentration dependant rates of hydrolysis of BAPNA are essentially identical for TrypZean™ and bovine trypsin.
Availability:
Research Sizes: 10mg and 100mg
Bulk: Contact Sigma-Aldrich® Fine Chemicals
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