Specificity:

Preferential cleavage: Phe-Xaa > Tyr-Xaa > Trp-Xaa > Leu-Xaa

Synthethic Substrates:

BTEE, B6125

Specificity:

Preferential cleavage: Ty-Xaa, Trp-Xaa, Phe-Xaa, Leu-Xaa

Synthethic Substrates:

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide, S7388
N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, S9761
Ala-Ala-Phe-7-amido-4-methylcoumarin, A3401
ALA-ALA-VAL-ALA P-Nitroanilide, A9273
BTEE, B6125
N-Benzoyl-L-tyrosine p-nitroanilide , B6760
Glutaryl-Ala-Ala-Phe-4-methoxy-b-naphthylamide, G3769
N-Glutaryl-Gly-Gly-Phe-b-naphthylamide, G4636
4-Methylumbelliferyl p-trimethylammoniocinnamate chloride, M4507
N-Succinyl-Gly-Gly-Phe p-nitroanilide, S1899
N-Succinyl-L-phenylalanine p-nitroanilide, S2628
N-Succinyl-Ala-Ala-Pro-Phe 7-amido-4-trifluoromethylcoumarin, S5189
N-Succinyl-Ala-Ala-Pro-Phe 4-methoxy-2-naphthylamide, S5314

Inhibitors:

Z-L-Phe chloromethyl ketone, C9511
a2-Antiplasmin, A0914
Aprotinin, A1153
e-Amino-n-caproic acid, A2504
a1-Antitrypsin, A6150
e-Amino-n-caproic acid, A7824
4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride, A8456
a1-Antitrypsin, A9024
a1-Antchymotrypsin, A9285
Bromoenol lactone, B1552
Chymostatin, C7268
Diisopropyl fluorophosphate, D0879
Ecotin, E1398
N-Acetyl-eglin C, E7888
Gabexate mesylate, G2417
Leupeptin, L2023
Trypsin Inhibitor, T0256
Trypsin Inhibitor, T1886
N-Tosyl-L-phenylalanine chloromethyl ketone, T4376
Trypsin Inhibitor, T4385
Trypsin Inhibitor, T9253
Trypsin Inhibitor, T9378
Trypsin-chymotrypsin inhibitor, T9777

Specificity:

Preferential cleavage: Arg-Xaa , including Arg-Pro, but not Lys-

Synthethic Substrates:

BAEE, B4500

Inhibitors:

p-Chloromercuribenzoic acid Free Acid, C5913
Histatin 5, H6027
N a-p-Tosyl-L-lysine chloromethyl ketone hydrochloride, T7254

Specificity:

Digestion of native collagen in the triple helical region at Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'

Synthethic Substrates:

Z-Gly-Pro-Leu-Gly-Pro, C0627
Collagen Type I-FITC, C4361
Collagen Type II-FITC, C4486
Collagen-Fluorescein, C5858
Collagen-Fluorescein, C8008
N-(2,4-Dinitrophenyl)-Pro-Leu-Gly-Cys(Me)-His-Ala-D-Arg amide, D0296
FALGPA, F5135

Inhibitors:

5-(R,S)-T-trans-Cinnamido-7-methyl-4-oxo-octanoyl-L-prolyl-L-proline, C0360
Dichloromethylenediphosphonic acid disodium salt, D4434
Ecotin, E1398
EDTA, ED2SS
Isoamylphosphonyl-Gly-L-Pro-L-Ala, dipotassium, I7029
(2R)-2-Mercaptomethyl-4-methylpentanoyl-beta-(2-naphthyl)-Ala-Ala Amide, M3531

Specificity:

Selective cleavage of Lys(or Arg) Ile bond in complement subcomponent C1s

Inhibitors:

Complement C1 esterase inhibitor, C2412
Diisopropyl fluorophosphate, D0879
C1 Esterase inhibitor, E0518

Specificity:

Cleavage of Arg-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg) Lys bond in complement component C2 to form C2a and C2b: the "classical" pathway C3 convertase

Inhibitors:

Complement C1 esterase inhibitor, C2412
Diisopropyl fluorophosphate, D0879
C1 Esterase inhibitor, E0518

Specificity:

Selective cleavage of Arg-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor

Inhibitors:

Diisopropyl fluorophosphate, D0879

Specificity:

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage

Specificity:

Hydrolysis of proteins with broad specificity

Specificity:

Release of an N-terminal dipeptide, Xaa-Xbb Xcc-, from a polypeptide, preferentially when Xbb is Pro, provided Xcc is neither Pro nor hydroxyproline

Synthethic Substrates:

Gly-Pro-7-amido-4-methylcoumarin hydrobromide, G2761
Gly-Pro p-nitroanilide hydrochloride, G0513

Inhibitors:

Ile-Pro-Ile, I9759

Specificity:

Hydrolysis of proteins, including elastin. Preferential cleavage Val-Xaa > Ala-Xaa

Synthethic Substrates:

Boc-Ala-Pro-Nva p-chlorothiobenzyl ester, B2017
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-trifluoromethylcoumar, M4563
N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide, M4765
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, M9771

Inhibitors:

Aprotinin, A1153
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Eglin C fragment 60-63 methyl ester, E2017
N-Acetyl-eglin C, E7888
a2-Macroglobulin, M6159
Phenylmethylsulfonyl fluoride, P7626

Specificity:

Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-Xaa

Synthethic Substrates:

N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, M9771

Inhibitors:

a1-Antitrypsin, A6150
a1-Antitrypsin, A9024
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Ecotin, E1398
a2-Macroglobulin, M6159

Specificity:

Cleaves specifically at the carboxyl side of arginine residues. The enzyme has also been shown to cleave Lys-Lys and Lys-Arg bonds; all Arg-X bonds may not be hydrolyzed. This enzyme also exhibits esterase and amidase activities.

Synthethic Substrates:

TAME, T4626

Inhibitors:

Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159
N-a-p-Tosyl-L-lysine chloromethyl ketone Hydrochloride, T7254

Specificity:

Cleavage of Xaa-Asp, Xaa-Glu and Xaa-cysteic acid bonds

Specificity:

Preferential cleavage: Glu-Xaa, Asp-Xaa

Inhibitors:

Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159

Specificity:

Cleaves at the carboxyl side of lysyl residues. Lys-Pro and Lys-Glu bonds are also cleaved

Synthethic Substrates:

N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt, T6140

Inhibitors:

Aprotinin, A1153
Leupeptin, L2023
Diisopropyl fluorophosphate, D0879
N-a-p-Tosyl-L-lysine chloromethyl ketone Hydrochloride, T7254

Specificity:

Activation of trypsinogen by selective cleavage of Lys6-Ile bond

Synthethic Substrates:

Gly-Asp-Asp-Asp-Asp-Lys-b-naphthylamide, G5261

Inhibitors:

Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159

Specificity:

Selective cleavage of Arg-Thr and then Arg-Ile bonds in prothrombin to form thrombin

Synthethic Substrates:

N-Benzoyl-Ile-Glu-Gly-Arg p-nitroanilide hydrochloride, B2291
N-Benzoyl-Ile-Glu-Gly-Arg p-nitroanilide acetate salt, B7020
Boc-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride, B9760
Factor Xa chromogenic substrate, F3301
4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride monohydrate, M4149

Inhibitors:

a2-Antiplasmin, A0914
Antithrombin III, Human, A2221
Antithrombin III, Rat, A6397
Antithrombin III, Bovine, A9141
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Ecotin, E1398
Phenylmethylsulfonyl fluoride, P7626
Trypsin Inhibitor, T9003