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 Terminology and Physical Properties

Radiochemicals
 

Glossary of Radiochemical Terms
Radiochemical Stability and Storage
Radiochemical Units
Isotope Labelling
Tritium Half-Life Decay Chart


Glossary of Radiochemical Terms
Activity The number of radiochemical transformations that take place in a sample during a specified period of time.
Alpha (a) emission Particulate radiation consisting of fast moving helium nuclei (2 protons, 2 neutrons) produced by the disintegration of heavy nuclei of atomic number 52.
Autoradiography The method of producing a viewable image of radioactive areas using photographic (X-ray) film.
Background Naturally occurring low-level radioactivity that can be detected by a scintillation counter. It must be subtracted from the total count to obtain net counts of the sample.
Becquerel (Bq) A Standard International Unit of radioactivity, which is equal to 1 disintegration per second and equal to 2.7 x10-11Ci.
Beta (b) emission An electron ejected from a nucleus during radioactive transformation. Beta particles produced by a given nuclei have a range of initial energies from a maximum, which is characteristic of the nuclide.
Bremsstrahlung Electromagnetic radiation produced when an electrically charged particle, such as an electron, is slowed down by the electric field of an atomic nucleus.
Carrier-free A preparation of a radioisotope to which no carrier isotope has been added, and for which precautions have been taken to minimize contamination with other isotopes. Material of high specific activity is often loosely referred to as carrier-free, but more correctly this should be termed material of high isotopic abundance.
Curie A unit of radioactivity which is equal to 3.7 x 1010 Bq.
Efficiency Refers to a detector. It is the ratio of the number of radiations actually detected by the detector to the number of radiations emitted by the source.
Electron capture Radioactive transformation in which the nucleus absorbs an electron from an inner orbital. The remaining orbital electrons rearrange to fill the empty electron shell and in so doing energy is released as electromagnetic radiation at X-ray wavelengths and/or as electrons.
Electron volt (eV) A unit of energy equal to the kinetic energy acquired by an electron when accelerated through a potential difference of 1 volt.
Enantiomorph Either of two forms of a substance that are mirror images of each other.
Gamma (g) emission Electromagnetic radiation emitted by atomic nuclei, the wavelength is generally in the range 1 W 10-10 to 2 W 10-13 m.
Half-life The period of time in which the activity of a radionuclide decays to half of its initial value.
Isotopes Nuclides having the same atomic number but different mass numbers.
Isotope effect A phenomenon that can occur when two isotopes of the same atomic number (or their compounds) may have a detectable difference in their chemical and physical behavior.
Isotopic abundance The number of atoms of a particular isotope in a mixture of the isotopes of an element, expressed as a fraction of all the atoms of the element.
Labeled compound A compound in which an isotope is incorporated into the molecule. Go to Nomenclature of isotope labeling for more details.
Liquid Scintillation Counting A technique used for measuring radioactivity of carbon 14 and tritium compounds, as well as other isotopes. The compound is dissolved in a scintillation fluid (or cocktail) which allows the energy from the particles to be transferred to the cocktail and then emitted as light photons (scintillations) which can be detected on a scintillation counter.
Milliatom The atomic weight of an element in milligrams.
Millimole (mmol) The molecular weight of a compound in mg.
Nuclide A species of atom characterized by its mass number, atomic number, and energy state, provided that the mean life in the state is long enough to observe.
Positron The antiparticle of the electron, having the same mass but an equal and opposite charge. It is produced in certain decay processes.
Radioactive concentration The activity per unit volume, normally expressed in mCi/mL. Not the same as the specific activity.
Radioactivity The property of certain nuclides of emitting radiation by the spontaneous transformation of their nuclei.
Radiochemical A compound in which one or more of the atoms of the compound are present in a radioactive form.
Radiochemical purity Purity of a radioactive material--the proportion of the total radioactivity that is present in the stated isotopic form.
Radiolabelled compound A compound in which one or more of the atoms of a proportion of the molecules is replaced by a detectable radioactive isotope.
Radionuclide (radioisotopic) purity Of a radioactive isotope, the proportion of the total radioactivity that is present in the stated isotopic form.
Scintillation The term used to describe light flashes emitted by luminescent substances when excited by high energy radiation.
Scintillation counter An instrument used to measure the scintillations emitted by a radioactive substance. Used to determine such things as radioactive concentration or specific activity.
Specific activity The radioactivity per unit mass of an element or compound containing a radioactive nuclei. Normally expressed as millicuries per milligram, millicuries per millimole, or as curies per millimole.
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Radiochemical Stability and Storage

Stability (shelf-life) of radiolabeled compounds is determined by a combination of the chemical and radiochemical decomposition processes.Radiochemical compounds with high specific activities are more likely to undergo radiation-induced chemical degradation. Tritium products are generally less stable than the carbon 14 products.This is due to the fact that tritium compounds usually have a much higher specific activity and are generally stored at low chemical concentrations (mg/mL) in solution.This is particularly a problem for aqueous and saline solutions, where non-sterile conditions can lead to microbial degradation.

Proper storage of radiochemical compounds is important.We provide product information sheets which will give the recommended storage conditions for our products.Our products are rigorously tested, and then are re-analyzed approximately every six months to ensure that the purity specifications are met.This provides you with the highest quality product available.General methods for analysis include HPLC, GC, and gel electrophoresis with electronic radioimaging, with HPLC being the preferred method of analysis.

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Radiochemical Units

Sigma's radiochemicals are sold by units of radioactivity, not by weight. The basic unit of radioactivity used in this catalog is the Curie (Ci) which is defined as "the quantity of any radioactive nuclide which undergoes 3.7 x 1010 disintegrations per second."

The Becquerel is the basic unit of radiation measurement according to the International Commission of Radiation Units and Measurements. For those who prefer to use the International System, the following conversion table is useful.


To Convert From To Multiply By
Becquerel (Bq) Curie (Ci) 2.7 x 10-11
Curie (Ci) Becquerel (Bq) 3.7 x 1010
Microcurie (µCi) Kilobecquerel (kBq) 37
Kilobecquerel (kBq) Microcurie (µCi) 0.027
Millicurie (mCi) Megabecquerel (MBq) 37
Megabecquerel (MBq) Millicurie (mCi) 0.027
Gray (Gy) Rad 100
Rad Gray (Gy) 0.01
Sievert (Sv) Rem 100
Rem Sievert (Sv) 0.01
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Isotope Labelling

Specifically labeled compounds are those in which all the isotopically labeled positions are identified and the radioactivity at these positions is greater than 95%. An example is Ethanol-2-14C, indicating that greater than 95% of the 14C present is on the number two carbon.

Uniformly Labeled Compounds (UL), usually carbon 14, are those in which all positions have been isotopically labeled to an equal degree. An example of a uniformly labeled 14C product would be Benzene-UL-14C.

Nominally labeled products (N) contain a significant amount of the label at the indicated position(s) but the extent of isotopic labeling at other positions is unknown. An example of a nominally labeled product would be 2-Deoxy-d-Glucose-1,2-3H(N).

Generally labeled compounds (G), usually tritium labeled, are those in which there is a general but not uniform distribution of the label among the available sites. An example of a generally labeled compound would be Riboflavin-[3H(G)].

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Tritium Half-Life Decay Chart
Tritium Half-Life Decay Chart
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