Specification Profiles

Biotinylated Biomolecule Capture

SigmaScreen™ Streptavidin Coated Plates

Description		Biotin Binding Capacity	%CV well to well	%CV plate to plate	DNase	Protease
96-well clear, M 5432 96-well white, M 5557		>25 pmole biotin per well	<10%	<10%	None detected	None detected
96-well black, M 4058		>25 pmole biotin per well	<12%	<12%	None detected	None detected
384-well clear, S 8686 384-well white, S 8561 384-well black, S 8811		>4 pmole biotin per well	<10%	<10%	None detected	None detected

SigmaScreen™ Streptavidin High Capacity Coated Plates

Description		Biotin Binding Capacity	%CV well to well	%CV plate to plate	DNase	Protease
96-well clear, S 6940		>300 pmole biotin per well	<15%	<15%	None detected	None detected
96-well black, S 8940		>200 pmole biotin per well	<15%	<15%	None detected	None detected
96-well white, S 9065		>250 pmole biotin per well	<15%	<15%	None detected	None detected
384-well clear, S 8815		>150 pmole biotin per well	<15%	<15%	None detected	None detected
384-well black, S 9190		>100 pmole biotin per well	<15%	<15%	None detected	None detected
384-well white, S 9315		>125 pmole biotin per well	<15%	<15%	None detected	None detected

SigmaScreen™ ExtrAvidin Coated Plates

Description		Biotin Binding Capacity	%CV well to well	%CV plate to plate	DNase	Protease
96-well clear, E 3027		>25 pmole biotin per well	<10%	<10%	None detected	None detected
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Cell-Based Assays

SigmaScreen™ Poly-D-Lysine Coated Plates

Description		Bioburden	Endotoxin	Plate Performance
96-well clear, M 3555 96-well white, M 5682 96-well black, M 5307		<0.52% wells contaminated (<10 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Tested wells show >2X signal of negative control wells. Visual confirmation of greater number of cells (BHK 21) adhered to test wells than negative control wells.
384-well clear, S 7436 384-well white, S 7561 384-well black, S 7686		<0.52% wells contaminated (<40 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Tested wells show >1.5X signal of negative control wells. Visual confirmation of greater number of cells (BHK 21) adhered to test wells than negative control wells.

SigmaScreen™ Collagen I Coated Plates

Description		Bioburden	Endotoxin	Plate Performance
96-well clear, S 8813 96-well white, S 3315 96-well black, S 3190		<0.52% wells contaminated (<10 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Test wells show approximately 90% of HT 1080 cells spread, as compared to negative control wells which demonstrate rounded cellular morphology and poor spreading.
384-well clear, S 3440 384-well white, S 3565 384-well black, S 3690		<0.52% wells contaminated (<40 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Test wells show approximately 90% of HT 1080 cells spread, as compared to negative control wells which demonstrate rounded cellular morphology and poor spreading.
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Epitope Tag and Protein Capture Plates

HIS-Select™ HC Nickel Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Capacity
96-well clear, S 5563		<15%	<15%	>4 5g protein per well
384-well clear, H 1661		<15%	<15%	>2 5g protein per well

HIS-Select™ HS Nickel Coated Plate

Description		%CV well to well	%CV plate to plate	Minimum Detectable	Protein Binding
96-well strip, S 5688		<10%	<10%	Absorbance of 1 ng/well	>0.20

Anti-FLAG™ HS M2 Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Sensitivity
96-well clear, P 2983		<10%	<10%	Detection of <1 ng of FLAG-BAP-His Control Protein above background

Protein G HS Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Sensitivity (signal/noise ratio)
96-well clear, S 2063		<10%	<10%	>1.5 (test wells show detection of an antibody Alkaline Phosphatase conjugate of <1 ng above background)

Glutathione Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Capacity	Binding Sensitivity
96-well clear, P 3233	n/a	<10%	<10%	>10 ng purified GST	<1 ng purified GST detected
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