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Glassware Catalog
TLC Chromatography
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TLC Chromatography
On this page: Plates • Accessories
Sigma-Aldrich TLC Plates
Thin-layer chromatography (TLC) plates for adsorption, partition, and ion exchange techniques. Six media choices with glass, polyester, and aluminum backings. Silica-based adsorbent layers in Sigma-Aldrich TLC plates incorporate a polymeric binder; highly purified silica gel plates incorporate a gypsum/polymer binder.
- Silica gel - for weakly to strongly polar separations
- High purity silica gel - acid washed for separation of aflatoxins
- Cellulose - for partition chromatography
- Cellulose PEI - for separation of weak anions (amino acids, peptides)
- Aluminum oxide - basic alumina, for separation of hormones and antibiotics
- Chiral silica - for separation of optically active isomers by ligand exchange
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Adsorbent layer
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Plate material
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Plate dimensions
(cm x cm)
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Fluorescent
indicator
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Catalog
Number
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| Layer thickness: 200 mm, particle size <60 mm, pore size 60Å |
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Aluminum oxide
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polyester
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20 x 20
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Y
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Aluminum oxide
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polyester
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20 x 20
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-
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Layer thickness: 250 mm, particle size 2 to 20 mm
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Cellulose
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glass
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10 x 20
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-
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Cellulose
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glass
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10 x 20
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Y
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Cellulose
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glass
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20 x 20
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-
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Cellulose
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glass
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20 x 20
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Y
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Layer thickness: 100 mm, particle size 2 to 20 mm
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Cellulose
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polyester
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20 x 20
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-
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Cellulose
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polyester
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20 x 20
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Y
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Cellulose, PEI*
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polyester
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20 x 20
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Y
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Layer thickness: 200 mm, particle size 2 to 25 mm, pore size 60Å
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Silica gel
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aluminum
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15 x 10
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Y
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Silica gel
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aluminum
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10 x 20
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Y
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Silica gel
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aluminum
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20 x 20
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Y
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Layer thickness: 250 mm, particle size 5 to 17 mm, pore size 60Å
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Silica gel
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glass
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15 x 20
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Y
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Silica gel
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glass
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15 x 20
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Y
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Silica gel
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glass
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10 x 20
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-
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Silica gel
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glass
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10 x 20
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Y
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Silica gel
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glass
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20 x 20
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-
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Silica gel
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glass
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20 x 20
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Y
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Silica gel
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polyester
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20 x 20
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-
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Silica gel
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polyester
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20 x 20
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Y
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Silica, highly purified
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glass
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20 x 20
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-
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Silica, highly purified
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glass
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20 x 20
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Y
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Silica, highly purified
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polyester
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20 x 20
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-
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Silica, highly purified
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polyester
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20 x 20
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Y
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| Layer thickness: 250 mm, particle size 2 to 10 mm, pore size 60Å |
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Chiral-silica gel
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glass
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15 x 20
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-
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Chiral-silica gel
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glass
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10 x 20
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-
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Chiral-silica gel
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glass
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20 x 20
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-
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*PEI plates may turn slightly yellow, affecting separation. Predevelop plates in distilled water to minimize yellow color.
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ACCESSORIES
TLC development chambers
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Uses only 2mL of solvent with elution complete in about 45s (using 20% EtOAc/hexanes as eluant). Greater resolution may be achieved by extending elution time. Borosilicate glass. Use with 2.5 x 7cm flexible plastic or glass TLC plates that may be spotted with up to 10 fractions 7mm apart along the edge of the plate.
Performance features:
- Rapid analysis
- Highly efficient use of TLC plate
- Very little solvent required
- Unlike beakers and jars, tube wall provides a straight base for even solvent flow
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No. slots
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Dim. L x o.d. (mm)
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Catalog Number
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Single
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95.9 x 25.4
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Dual
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...190 x 25.4 .
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| TLC Plates, silica gel; 2.5 x 7.5cm glass plates, 250 mm layer. Package of 100 |
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1) Levine, S.G., J. Chem. Educ.,1996,73,77.
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TLC developing tanks
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Can be used as a staining chamber. Made of glass with lid.
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O.d. x H (cm)
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Catalog Number
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6.5 x 10.5
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6.5 x 21.0
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| Replacement tank lid |
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TLC developing tanks
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Glass with lid.
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Inside dim. (cm)
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Complete
Catalog Number
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Lid Only
Catalog Number
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27.5 x 27.5 x 7.5
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17.5 x 16.0 x 6.2
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17.5 x 16.0 x 8.2
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17.5 x 11.0 x 6.2
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17.5 x ..6.2 x 6.8
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12.1 x 10.8 x 8.3
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7.5 x 15.5 x 8.0
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TLC spotting guide and Rf reader
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Markings at the bottom of the template are useful guides for applying samples at uniform distances across the plate. The guide is printed on 5mm clear acetate and measures 7 x 8 1/2in. (180 x 215mm).
S 2009
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ALDRICH CHROMATOGRAPHY SPRAYERS
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Provide a fine, uniform spray that is optimized for the development of TLC plates. Also suited for use in electrophoresis.
- Adjustable spray pattern using thumb on vent hole
- Greaseless, screw-threaded ST joint will not seize; a simple turn of the threaded cap pulls joint apart safely
- Threaded hose barb prevents accidental breakage during tubing installation or removal
- Uses low-pressure gas or air (<5psi).
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Flask size (mL)
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Catalog Number
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10
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50
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125
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250
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Compressed-air sprayers for spraying TLC plates. Apparatus includes a glass-sprayer head and the designated tube, bottle, or flask.
 Bottle-type sprayer
 Flask-type sprayers
Erlenmeyer flask with #15 Ace-Thred with sprayer head.
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Cap. (mL)
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Catalog Number
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175
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250
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| Replacement parts |
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| Erlenmeyer flask with #15 Ace-Thred. |
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175
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250
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| Sprayer head |
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Kimble micro capillary pipettes
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Disposable pipettes are calibrated "to contain". Pipettes are 5 inches long and the different sizes are color-coded. Made of soda lime glass. Each cylinder pack comes with one pipette device. Filling is easy. Capillary action draws the fluid into the tube. To dispense, simply cover the hole on the rubber bulb and squeeze.
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Cap. (mL)
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Tol. (±)
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Catalog Number
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5
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1.0
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10
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0.5
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20
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0.5
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25
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0.5
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50
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0.5
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100
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0.5
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