Whatman Blotting Membrane Selection Guide

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Whatman Membrane Selection

Whatman Blotting Membrane Selection Guide
	Membranes & Blotting Paper

Use the blotting membrane comparison table below to select the most appropriate Whatman membrane for your application. Click on the transfer membrane type to view list of available products.
TRANSFER MEMBRANES
	Protran^®	Optitran^®	Nytran^® N	Nytran^® SuPerCharge	Westran^® S	Westran^® Clear Signal
FEATURE:	Universal nitrocellulose blotting membrane. Superior for chemiluminescence detection using horseradish peroxidase.	Nitrocellulose membrane with reinforcing fleece. Suitable for repeated probing.	Nylon membrane for nucleic acid applications. Easier to block than Nytran SuPerCharge.	Nylon membrane for nucleic acid applications. Highest binding capacity available.	PVDF membrane for protein blotting, designed for protein sequencing. Better binding low MW proteins.	PVDF membrane for protein blotting. Specifically designed for Western blotting.
MEMBRANE TYPE:	Nitrocellulose, 100% pure	Nitrocellulose, reinforced	Nylon, moderately positively charged	Nylon, highly positively charged	PVDF	PVDF
APPLICATIONS:	Western, Southern, Northern blotting	Western, Southern, Northern blotting	Southern, Northern blotting	Southern, Northern blotting	Western blotting, Sequencing	Western blotting
BINDING:	75-110 µg/cm²	75-90 µg/cm²	>400 µg/cm²	>600 µg/cm²	>50-100 µg/cm²	>50-100 µg/cm²
PORE SIZES:	0.45 µm 0.2 µm 0.1 µm	0.45 µm 0.2 µm -	0.45 µm 0.2 µm -	0.45 µm - -	- 0.2 µm -	0.45 µm - -
TRANSFER METHODS
Semi-dry Blotting	++	++	++	++	++	++
Tank Blotting	++	++	++	++	++	++
Vacuum Blotting	++	++	++	++	+	+
Capillary Blotting	++	++	++	++	+	+
Alkaline Method	not recommended	not recommended	++	++	not recommended	not recommended
IMMOBILIZATION
UV-crosslinking, DNA, RNA	++	++	++	++	-	-
Baking (80 °C), DNA, RNA	++	++	+	+	-	-
Drying, DNA, RNA	-	-	+	+	-	-
Drying, Protein	++	++	-	-	++	++
DETECTION METHODS
Colorimetric	++	++	+	+	++	++
Chemiluminescent	++	++	++	++	++	++
Isotopic	++	++	++	++	++	++
Fluorescent	++	-	-	-	-	-
REPROBING	limited	++	++	++	++	++
++ Recommended + Satisfactory

Membranes with smaller pore sizes are generally recommended for the transfer of smaller molecules. A 0.2 µm pore size ensures high retention of proteins below 20 kD. For molecules below 7 kD, a 0.1 µm pore size is recommended.
Product Lists: Protran^® Optitran^® Nytran^® N Nytran^® SuPerCharge Westran^® S Westran^® Clear Signal

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