Antibody Explorer Home Kits

Sigma offers a variety of unique kits to support immunochemistry and immunodetection applications including immunogenicity enhancement, antibody purification and isotyping and probe signal amplification.

The study of the proteosome is a rapidly evolving field that frequently requires dissection of structural proteins, enzymes and cell signaling molecules into functional or structural domains involving relatively short amino acid sequences. Very often these peptides (haptens) are not immunogenic unless they are conjugated to larger molecules such as bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH). Sigma offers antibody conjugations kits that contain maleimide activated BSA and KLH which support the induction of immunogenicity of small sulfhydryl containing haptens.

Sigma provides kits to facilitate purification of polyclonal and monoclonal antibodies and epitope-tagged proteins (FLAG tagged). These kits utilize the antibody binding features of protein A to facilitate antibody purification from serum, ascites or cell culture supernatants and protein G for purification of antibodies immunoprecipitated (IP) from complex cell lysates. FLAG tagged proteins are recovered using a high affinity ANTI-FLAG M2 antibody agarose matrix.

When choosing detection reagents or developing a purification scheme it is generally useful to know the target antibody’s isotype or subclass. We offer isotyping kits for determination of an antibody’s subclass with simple immunodiffusion (Ouchterlony) or ELISA assays.

The use of avidin biotin based probes for immunoassay detection systems is a well established method for enhancing (amplifying) probe signals. We offer avidin-biotin based alkaline phosphatase and peroxidase system that utilizes a unique form of avidin, ExtrAvidin® that combines high specificity and affinity for avidin with low non-specific binding at physiological pH.