Product List for "Removal of Cross-reactive Antibodies from Antiserum: Affinity  Chromatography" Protocol

The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in a partnership with Sigma-Aldrich. A list of products is available to use in each protocol. View the protocol on BioSupplyNet.com.

Selected Protocol from "Molecular Cloning: A Laboratory Manual," Third Edition, Joseph Sambrook, David W. Russell. Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography Joseph Sambrook Peter Maccallum Cancer Institute and The University of Melbourne, Australia David W. Russell University of Texas Southwestern Medical Center, Dallas ABSTRACT This protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of immunoglobulins through a column containing immobilized bacterial proteins.

Buy this book, M8265

Products Available for this Protocol

Protocol Material Description	Product #	Product Name	Add to Cart
Sodium borate (pH 8.0)	B9876	Sodium tetraborate decahydrate, ReagentPlus®, 99.5–105.0%
NaCl	S3014	Sodium chloride, for molecular biology, ≥98% (titration)
Pancreatic DNase I	D4527	Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein ~90 %, ≥2,000 units/mg protein
Tris-Cl (pH 7.5)	T5941	Trizma® hydrochloride, for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration)
MgCl2	M4880	Magnesium chloride, insect cell culture tested
NaOH	S8045	Sodium hydroxide, SigmaUltra, ≥98%, pellets (anhydrous)
TBS	T5912	Tris Buffered Saline, 10X, solution
Sodium azide	S8032	Sodium azide, SigmaUltra
Triton X-100	234729	Triton® X-100

Back to Top
View all Cold Spring Harbor Antibody Protocols
View all available Cold Spring Harbor Laboratory Press Protocols

Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.