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Yeast Chromatin Immunoprecipitation (ChIP) Assay
William P. Tansey
ABSTRACT
This protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of this assay.
Products Available for this Protocol
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Buffers,
Solutions, and Reagents |
|
|
|
| Antibody of choice |
Visit the Antibody Explorer for all your antibody needs. |
| NaCl |
S3014 |
Sodium chloride, for molecular biology |
|
| NP-40 |
NP40S |
Tergitol® solution, Type NP-40, 70% in H2O |
|
| Deoxycholate (DOC) |
D6875 |
21-Hydroxyprogesterone |
|
| SDS (10%) |
L4390 |
Sodium dodecyl sulfate, for molecular biology |
|
| Tris-Cl |
T5941 |
Trizma® hydrochloride, for molecular biology, Biotechnology
Performance Certified |
|
| Aprotinin |
A6103 |
Aprotinin from bovine lung, recombinant, expressed in
Nicotiana |
|
| Pepstatin |
P5318 |
Pepstatin A |
|
| Leupeptin |
L9783 |
Leupeptin hydrochloride |
|
| Pefabloc |
76307 |
Pefabloc® SC |
|
| LiCl |
L9650 |
Lithium chloride, for molecular biology |
|
| Ethanol |
E7023 |
Ethanol, 200 proof (absolute), for molecular biology |
|
| Glycine |
G8790 |
Glycine, meets EP, JP, USP testing specifications, cell culture
tested |
|
| Glycogen |
G1767 |
Glycogen from Mytilus edulis (Blue mussel) |
|
| NaOAc |
S5636 |
Sodium acetate, cell culture tested, insect cell culture
tested |
|
| Paraformaldehyde (PFA) |
158127 |
Paraformaldehyde, powder, 95% |
|
| Phenol:chloroform |
P1944 |
Phenol:Chloroform 5:1, for molecular biology |
|
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