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Genomic testing and characterization has become an important tool for understanding biological systems. Often, such analysis is hampered by the number of samples to be examined and the availability of sufficient quantities of genomic DNA. This is particularly a problem for rare and archived sources of DNA. The GenomePlex® Whole Genome Amplification (WGA) kits have been developed for use as a high-throughput system for the rapid and highly representative, amplification of genomic DNA from trace amounts of starting material (Figure 1 and Figure 2).
The GenomePlex® WGA kits utilize a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex® Library molecules flanked by universal priming sites. The OmniPlex® library is then amplified using universal oligonucleotide primers and a limited number of cycles. The GenomePlex® WGA kits require nanogram quantities of genomic DNA with yields of 5-10 µg following PCR. This system is suitable with a variety of purified DNA samples including whole blood, blood cards, buccal swabs, plant sources, and formalin-fixed, paraffin-embedded tissue samples (Figure 3).
Applications
Automation of whole genome amplification using the GenomePlex® kit is suitable for a variety of applications including:
- TaqMan® assays
- Microsatellite analysis
- SNP analysis
- Sequencing
- CGH analysis
- Microarray analysis
Features & Benefits
Rapid Procedure
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96 samples can be prepared for WGA and amplified in under 4 hours using the Sciclone ALH 3000 Workstation.
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Compatibility
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The automated method for GenomePlex® WGA is suitable for use with a variety of source samples including: buccal swabs, whole blood, blood cards, plant tissue, and formalin-fixed, paraffin-embedded tissues.
Genomic DNA amplified using GenomePlex® WGA can be used in a variety of downstream applications including TaqMan assays, SNP analysis, sequencing, CGH analysis, and microarray analysis.
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Immortalizes Source Samples
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Nanogram quantities of genomic DNA can be amplified up to 500-fold.
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Representative Amplification
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WGA is accomplished with minimal allelic drop out.
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Reproducible
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The automated GenomePlex® WGA method provides accurate reaction setup, while eliminating human error and cross contamination.
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| GenomePlex® Whole Genome Amplification Kits |
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| Product # |
Product Name |
Package Size |
| WGA1* |
GenomePlex® Whole Genome Amplification Kit |
50 Reactions |
| WGA2* |
GenomePlex® Complete Whole Genome Amplification Kit |
50 Reactions |
* To perform WGA on a 96-well plate of samples, 3 kits must be ordered. Alternatively, custom packaging is available. Contact your local Sigma sales representative for more information.
Kit Contents
- Fragmentation Buffer
- Library Preparation Buffer
- Library Stabilization Solution
- Library Preparation Enzyme
- 10x Amplification Master Mix
- Nuclease-free Water
- Control Human Genomic DNA
- WGA DNA Polymerase (WGA2 kit only)
| Supporting Reagents |
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| Product # |
Product Name |
Package Size |
| WGA3* |
GenomePlex® WGA Reamplification Kit |
50 Reactions |
* To perform WGA on a 96-well plate of samples, 3 kits must be ordered. Alternatively, custom packaging is available. Contact your local Sigma sales representative for more information.
Whole Genome Amplification: Formalin-fixed, Paraffin-embedded Tissue
Figure 1. 10 ng of DNA isolated from 88 samples of formalin-fixed, paraffin-embedded (FFPE) rat liver tissue were amplified using the automated WGA procedure on the Sciclone ALH 3000 workstation. 6 µl of each amplified product were analyzed on a 1% agarose gel. M: PCR marker. (+): Human genomic DNA control. (-): No DNA template control.
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Cross Contamination Analysis
Figure 2. 10 ng of human genomic DNA samples or 8 µl of water were placed in alternating wells of an amplification plate. The plate was processed using the automated WGA procedure on the Sciclone ALH 3000 workstation. All samples were then subjected to amplification and 6 µl of the resultant products were electrophoresed on a 1% agarose gel. Amplified products were not observed in the wells containing water.
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Whole Genome Amplification: Different Sources
Figure 3. DNA isolated from buccal swab, tomato leaves, whole blood, blood card, and FFPE rat liver tissue were amplified using the automated WGA procedure on the Sciclone ALH 3000 workstation. 6 µl of each amplified product were analyzed on a 1% agarose gel.
GenomePlex and OmniPlex are registered trademarks of Rubicon Genomics, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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