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Azoxymethane
Prod. No. A2853 |
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Azoxymethane (AOM) is a potent carcinogen used to induce colon cancer in rats and mice. It has been used in studies evaluating efficacy of preventative treatment for azoxymethane-induced carcinogenesis[1,2,3]. Azoxymethane is also commonly used to determine the chemopreventative effectiveness of particular foods such as undigestable sugars[4,5], red meat[6], and green tea[7] among others in rodent models. These rodent model results aid in the identification of possible preventative approaches to human colon cancer[8].
Changes or abnormalities in transforming growth factor beta (TGF-b) signaling are detected in tumors developed by mice treated with AOM[9,10]. Treatment with azoxymethane activates intrinsic tyrosine kinase of EGF receptor while stimulating the synthesis of TGF-alpha[11].
The cyclooxygenase 2 (COX-2) inhibitor NS-398 (N-194) reduces the incidence of preneoplastic cells in rats treated with azoxymethane[12].
Sigma is pleased to make this important compound available to cancer researchers.
References:
- Escribano, M., et al., Aspirin inhibits endothelial nitric oxide synthase (eNOS) and Flk-1 (vascular endothelial growth factor receptor-2) prior to rat colon tumour development. Clin. Sci. (Lond)., 106: 83-91 (2004).
- Marotta, F., et al., Chemopreventive effect of a probiotic preparation on the development of preneoplastic and neoplastic colonic lesions: an experimental study. Hepatogastroenterology, 50: 1914-8 (2003).
- Orii, S., et al., Chemoprevention for colorectal tumorigenesis associated with chronic colitis in mice via apoptosis. J. Exp. Clin. Cancer Res., 22: 41-6 (2003).
- Pool-Zobel, B., et al., Experimental evidences on the potential of prebiotic fructans to reduce the risk of colon cancer. Br. J. Nutr., Suppl 2: S273-81 (2002).
- Nakanishi, S., et al., Effects of high amylose maize starch and Clostridium butyricum on metabolism in colonic microbiota and formation of azoxymethane-induced aberrant crypt foci in the rat colon. Microbiol Immunol., 47: 951-8 (2003).
- Pierre, F., et al., Meat and cancer: haemoglobin and haemin in a low-calcium diet promote colorectal carcinogenesis at the aberrant crypt stage in rats. Carcinogenesis, 24: 1683-90 (2003).
- Metz, N., et al., Suppression of azoxymethane-induced preneoplastic lesions and inhibition of cyclooxygenase-2 activity in the colonic mucosa of rats drinking a crude green tea extract. Nutr Cancer., 38: 60-4 (2000).
- Corpet, D.E. and Pierre, F., Point: From animal models to prevention of colon cancer. Systematic review of chemoprevention in min mice and choice of the model system. Cancer Epidemiol. Biomarkers Prev., 12: 391-400 (2003).
- Guda, K., et al., Defective processing of the transforming growth factor-beta1 in azoxymethane-induced mouse colon tumors. Mol. Carcinog., 37: 51-9 (2003).
- Guda, K., et al., Aberrant transforming growth factor-beta signaling in azoxymethane-induced mouse colon tumors. Mol Carcinog., 31: 204-13 (2001).
- Relan, N.K., Identification and evaluation of the role of endogenous tyrosine kinases in azoxymethane induction of proliferative processes in the colonic mucosa of rats. Biochim Biophys Acta., 1244(2-3): 368-76 (1995
Jun 9)
- Kishimoto, Y., et al., Effects of cyclooxygenase-2 inhibitor NS-398 on APC and c-myc expression in rat colon carcinogenesis induced by azoxymethane. J. Gastroenterol., 37: 186-93 (2002).
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