Catalog CHO Medium Products

As many researchers have discovered, there is no one medium that grows all CHO clones equally well. Because of this, Sigma-Aldrich offers the widest selection of serum-free medium in the industry to allow you to select the ones that work best for your particular clones and applications. Our available catalog formulations include serum-free, animal-component free and chemically defined formulations that are designed to promote outstanding viable cell growth and recombinant protein productivity under a variety of conditions.

This medium is optimized for the production of recombinant proteins and is recommended for the growth of CHO cells in suspension and attached conditions for research and diagnostic applications. This formulation contains inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, and vitamins, as well as small quantities of animal components (fetuin, insulin, BSA, transferrin).

This animal-component free medium was developed to meet the needs of biotechnology and vaccine manufacturing and supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods, resulting in increased productivity. This formulation contains inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.

Also designed for biopharmaceutical applications, this new animal-component free medium is formulated to provide outstanding cell growth and recombinant protein production in CHO cells using the Dihydrofolate Reductase (DHFR-) gene amplification system. This formulation contains inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.

For further information on the development of this medium, please view our Poster Gallery.

This animal-component free medium is formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells in adherent culture systems. This formulation contains inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, recombinant human EGF, plant hydrolysates, other organic compounds, and trace elements.

For further information on the development of this medium, please view our Poster Gallery.

Formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells, this animal-component free, chemically defined medium maintains the functional properties of good cell growth and protein expression in cultured cells. This formulation contains no hydrolysates or other undefined components and is composed of inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, other organic compounds, trace elements, and surfactants.

Our newest animal-component free chemically defined medium is formulated to promote excellent cell growth and protein productivity in Chinese hamster ovary (CHO) cells. Developed to meet the needs of biopharmaceutical manufacturing, this medium supports rapid initial cell growth and maintains high levels of protein expression in suspension cultures for extended periods. This formulation does not contain hydrolysates or other undefined components and is composed of inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, other organic compounds, trace elements, and surfactants.

For further information on our family of serum-free CHO media, please view our Poster Gallery.