CHO Supplements

Sigma-Aldrich offers a range of medium supplements and reagents to help you optimize productivity in your CHO systems. Designed with the biopharmaceutical industry in mind, these animal-component free products are manufactured in large scale under cGMP.

Animal-component free feed concentrate formulated to optimize CHO cell growth and recombinant protein production in fed-batch bioreactor cultures.

The formulation includes amino acids, vitamins, recombinant human insulin, plant hydrolysates, trace elements and other organic compounds.

Concentrated carbonate/bicarbonate solution designed specifically to adjust pH in bioreactor applications. Using this buffered solution to adjust the pH in bioreactors minimizes detrimental localized pH effects that can occur when using NaOH and reduces the osmolality impact when compared to using NaOH.

Animal-component free. Proprietary aqueous mixture of fatty acids that have been found to be optimal for cell growth and protein expression. Recommended for use in CHO cultures at a concentration of 0.25 ml/L to 0.5 ml/L of medium.

The glutamine synthetase (GS) system is used to express recombinant proteins from mammalian cells. Glutamine-dependent cells transfected with a glutamine synthetase gene can be selectively grown in a suitable glutamine-deficient medium. GSEM Supplement is designed to improve cell growth and productivity in GS systems. Recommended for use in CHO cultures at a concentration of 20 ml/L of medium.

Animal-component free protein hydrolysate that is a rich source of L-glutamine. Recommended for CHO medium and feed supplementation at 1 to 5 g/L of medium.
_{HyPep 4601 is a trademark of Quest International.}

Further Information on our selection of CHO supplements.