Monoclonal antibodies
produced in mammalian cell culture systems are becoming increasingly important for the treatment of human
disease. Today, therapeutically useful monoclonal antibodies are produced either by hybridoma
cells, or as recombinant proteins in cell lines such as those derived from Chinese Hamster Ovary (CHO) cells. Please see our CHO media web-page for more details.
Traditional techniques for monoclonal antibody production using hybridoma cells have relied upon the use of medium,
such as Iscove's Modified Dulbecco's Medium (IMDM), supplemented with various
components (insulin, transferrin, selenium, pyruvate) and/or bovine sera. In the beginning, monoclonal
antibodies were employed primarily for research or in vitro diagnostic applications, and there was only limited incentive to invest time and money in
the elimination of serum as a supplement.
However, as new technologies have developed, cell culture produced monoclonal antibodies
have become important as potential in vivo human therapeutic agents. These changes in the intended use of monoclonal antibodies have led
to new concerns about the materials and methods employed for monoclonal antibody
production. The threat of contamination of the medium, and ultimately the
final therapeutic drug by adventitious agents extant in animal-derived materials, has led many regulatory agencies to strongly recommend the
discontinued, or limited use of serum and animal-derived materials. In
response to this situation, Sigma has developed several media for the growth and
maintenance of monoclonal antibody producing hybridoma cells which are serum-free, and/or animal-derived protein-free.
The table below lists the serum-free, animal-derived protein-free and chemically-defined
media which Sigma offers for use in the production, by hybridoma cells, of
therapeutically useful monoclonal antibodies. For more traditional media that
may be useful for producing monoclonal antibodies for in vitro research and diagnostics, please go to our classical media web-page for various formulations
of IMDM or RPMI-1640. Also, please see the hybridoma section of our Life Science catalogue.
|
|
|
back to top |
| Product # |
Product Name |
Description |
Application |
|
H 4409 |
Hybridoma Medium, Animal Components-free
Performance Data
|
- Liquid;
- Proprietary formulation, with light sensitive components. Protect from
light.
- Contains inorganic salts, sodium bicarbonate, essential and
non-essential amino acids, vitamins, recombinant human insulin, other
organic compounds, and trace elements.
- Without antibiotics, antimycotics, transferrin or L-glutamine;
supplement with 40 ml of 200 mM L-glutamine per liter of medium prior to
use.
- pH 7.1 to 7.7
- Sterile filtered;
- Endotoxin tested;
- Hybridoma tested.
|
This medium contains no animal derived components and is formulated for the growth of hybridoma cells that produce antibodies
suitable for use in therapeutic applications.
|
|
H 7662 |
Hybridoma Medium, Animal Components-free
|
- Powder;
- Proprietary formulation, with light sensitive components. Protect from
light.
- Contains inorganic salts, essential and
non-essential amino acids, vitamins, recombinant human insulin, other
organic compounds, and trace elements.
- Without antibiotics, antimycotics, transferrin or L-glutamine;
supplement with 40 ml of 200 mM L-glutamine per liter of medium prior to
use.
- pH 7.1 to 7.7
- Hybridoma tested.
|
This medium contains no animal derived components and is formulated for the growth of hybridoma cells that produce antibodies
suitable for use
in therapeutic applications.
|
|
H 4281 |
Hybridoma medium, Serum-free, Low-protein
Performance Data
|
- Liquid,
- Proprietary formulation, with light sensitive components. Protect from
light.
- Contains inorganic salts, sodium bicarbonate, essential and
non-essential amino acids, vitamins, recombinant human insulin, transferrin, bovine serum albumin, other
organic compounds, and trace elements.
- Without antibiotics, antimycotics, or L-glutamine;
supplement with 40 ml of 200 mM L-glutamine per liter of medium prior to
use.
- pH 7.1-7.7
- With L-glutamine and sodium bicarbonate;
- Sterile filtered;
- Endotoxin tested;
- Hybridoma tested.
|
Serum-free, low-protein medium formulated to support the growth of hybridoma
cells used in the manufacture of monoclonal antibodies. It supports the rapid growth of cells to high densities, and the maintenance
of viable cells at these high densities for extended periods of time. Hybridoma cells may be transferred without adaptation into this
medium from serum supplemented media.
Because hybridomas can be cultured in this medium without expensive and
chemically complex serum supplementation, down-stream purification is simplified and overall production costs are reduced. |
|
S 2897 |
Hybridoma Medium,
Serum-free,
Protein-free,
Chemically Defined
Hybri-MaxTM |
- Liquid;
- With L-glutamine, sodium bicarbonate and MOPS buffer.
- Sterile filtered;
- Endotoxin tested;
- Hybridoma tested
|
Complete
serum-free, protein-free medium formulated to support the growth of hybridoma
cells used in the manufacture of monoclonal antibodies.
The purification, of
monoclonal antibodies, which are produced by hybridoma cells, may be simplified
when grown in this chemically defined medium.
This medium is buffered with sodium bicarbonate and MOPS buffer. MOPS buffer has
a pKa = 7.2 with an effective buffering range from 6.5-7.9. It's inclusion in this medium increases buffer capacity and stability within the
optimal pH range when hybridoma cells are being maintained at high densities, and producing monoclonal antibodies.
This formulation eliminates regulatory concerns, about the risks
of biological contamination of recombinant therapeutics, that are associated with the use of serum or animal derived proteins.
|
|
S 8284 |
Hybridoma Medium,
Serum-free and Protein-free,
Chemically defined,
Hybri-MaxTM |
- Liquid;
- With L-glutamine, sodium bicarbonate and MOPS buffer.
- Without phenol red
- Sterile filtered;
- Endotoxin tested;
- Hybridoma tested.
|
Same as S 2897, except that phenol red has been removed.
Phenol red may be toxic
to some cells at clonal
densities. Use this medium when producing and cloning monoclonal
antibody producing hybridoma cells.
|
|
S 2772 |
Hybridoma Medium,
Serum-free and Protein-free,
Chemically defined,
Hybri-MaxTM |
- Powder;
- With L-glutamine and MOPS buffer;
- Without sodium bicarbonate: supplement with 2.25 g/L of sodium
bicarbonate.
- Formulated at 18.4 grams of powder per liter of medium.
- Endotoxin tested;
- Hybridoma tested;
|
Powder version of S 2897.
|
|
|
|
back to top |
Hybridoma monoclonal antibody expression systems are complex, and are typically modified
(or optimized) to meet the specific priorities and needs of the biotechnologist.
In addition to offering the above varieties of hybridoma media, Sigma provides
supplements that you may find useful in your culture system. The table below identifies some of the most useful supplements for your evaluation and use.
Other
supplements potentially useful for the culture of hybridoma cells such as transferrin, albumin, and insulin are offered by Sigma in many varieties.
These components are offered as individual reagents or in pre-formulated, ready
to use mixtures. Please use the Sigma search engine to find these product families.
Details
for the use of the reagents and supplements may be found in literature published by Sigma
which discusses the use of hybridoma cell lines for monoclonal antibody
manufacturing applications.
|
|
|
back to top |
| Product # |
Product Name |
Description |
Application |
|
A 1345 |
Anti-oxidant Supplement (1000x) |
- Liquid
- Sterile filtered.
- Endotoxin tested.
- Cell culture tested.
|
Proprietary medium supplement specially formulated to boost cell growth for enhanced protein expression. May be used in combination
with Polyamine Supplement (P 8483)
|
|
G 7513 |
L-Glutamine Solution, 200 mM |
- Liquid
- Sterile filtered.
- Endotoxin tested.
- Cell culture tested.
|
Prepared in cell culture grade water. Useful for supplementing liquid CHO media.
|
|
P 1300 |
Pluronic F-68,
Non-ionic Detergent |
- Liquid
- Cell culture tested.
|
A media additive often used to protect cells growing in suspended culture systems from damage which may occur during
agitation and aeration.
|
|
P 8483 |
Polyamine Supplement (1000x) |
- Liquid
- Sterile filtered.
- Endotoxin tested.
- Cell culture tested.
|
Medium supplement formulated to boost cell growth for enhancement of protein expression.
|
|
|
|
back to top |
|
){kind=link}
</b> is a low-protein medium formulated<br>
to support the growth of hybridomas used in the manufacture of monoclonal antibodies.
H 4281outperforms the competition.')){kind=link}