Coated Multiwell Plates Specification Profile

Sigma's line of coated plates for high throughput screening undergo extensive validation.
Each lot is assayed to ensure the exceptional performance and consistent quality
necessary to generate reliable results.
For detailed information regarding the assay performed, click on the Technical Bulletin icon:

    Biotinylated Biomolecule Capture
    Cell-Based Assays
    Epitope Tag and Protein Capture Plates

Biotinylated Biomolecule Capture

SigmaScreen™ Streptavidin Coated Plates

Description		Biotin Binding Capacity	%CV well to well	%CV plate to plate	DNase	Protease
96-well clear, M 5432 96-well white, M 5557		>25 pmole biotin per well	<10%	<10%	None detected	None detected
96-well black, M 4058		>25 pmole biotin per well	<12%	<12%	None detected	None detected
384-well clear, S 8686 384-well white, S 8561 384-well black, S 8811		>4 pmole biotin per well	<10%	<10%	None detected	None detected

SigmaScreen™ Streptavidin High Capacity Coated Plates

Description		Biotin Binding Capacity	%CV well to well	%CV plate to plate	DNase	Protease
96-well clear, S 6940		>300 pmole biotin per well	<15%	<15%	None detected	None detected
96-well black, S 8940		>200 pmole biotin per well	<15%	<15%	None detected	None detected
96-well white, S 9065		>250 pmole biotin per well	<15%	<15%	None detected	None detected
384-well clear, S 8815		>150 pmole biotin per well	<15%	<15%	None detected	None detected
384-well black, S 9190		>100 pmole biotin per well	<15%	<15%	None detected	None detected
384-well white, S 9315		>125 pmole biotin per well	<15%	<15%	None detected	None detected

SigmaScreen™ ExtrAvidin Coated Plates

Description		Biotin Binding Capacity	%CV well to well	%CV plate to plate	DNase	Protease
96-well clear, E 3027		>25 pmole biotin per well	<10%	<10%	None detected	None detected

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Cell-Based Assays

SigmaScreen™ Poly-D-Lysine Coated Plates

Description		Bioburden	Endotoxin	Plate Performance
96-well clear, M 3555 96-well white, M 5682 96-well black, M 5307		<0.52% wells contaminated (<10 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Tested wells show >2X signal of negative control wells. Visual confirmation of greater number of cells (BHK 21) adhered to test wells than negative control wells.
384-well clear, S 7436 384-well white, S 7561 384-well black, S 7686		<0.52% wells contaminated (<40 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Tested wells show >1.5X signal of negative control wells. Visual confirmation of greater number of cells (BHK 21) adhered to test wells than negative control wells.

SigmaScreen™ Collagen I Coated Plates

Description		Bioburden	Endotoxin	Plate Performance
96-well clear, S 8813 96-well white, S 3315 96-well black, S 3190		<0.52% wells contaminated (<10 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Test wells show approximately 90% of HT 1080 cells spread, as compared to negative control wells which demonstrate rounded cellular morphology and poor spreading.
384-well clear, S 3440 384-well white, S 3565 384-well black, S 3690		<0.52% wells contaminated (<40 wells out of 20 plates)	<0.5 EU/mL for all wells tested	Test wells show approximately 90% of HT 1080 cells spread, as compared to negative control wells which demonstrate rounded cellular morphology and poor spreading.

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Epitope Tag and Protein Capture Plates

HIS-Select™ HC Nickel Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Capacity
96-well clear, S 5563		<15%	<15%	>4 5g protein per well
384-well clear, H 1661		<15%	<15%	>2 5g protein per well

HIS-Select™ HS Nickel Coated Plate

Description		%CV well to well	%CV plate to plate	Minimum Detectable	Protein Binding
96-well strip, S 5688		<10%	<10%	Absorbance of 1 ng/well	>0.20

Anti-FLAG™ HS M2 Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Sensitivity
96-well clear, P 2983		<10%	<10%	Detection of <1 ng of FLAG-BAP-HIS Control Protein above background

Protein G HS Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Sensitivity (signal/noise ratio)
96-well clear, S 2063		<10%	<10%	>1.5 (test wells show detection of an antibody Alkaline Phosphatase conjugate of <1 ng above background)

Glutathione Coated Plate

Description		%CV well to well	%CV plate to plate	Binding Capacity	Binding Sensitivity
96-well clear, P 3233	n/a	<10%	<10%	>10 ng purified GST	<1 ng purified GST detected

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