Normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components. While some cells are able to synthesize these components, others require an exogenous source, particularly when grown in serum-free culture.
To help facilitate attachment, cell spreading, growth, morphology, differentiation, and motility of your cells, Sigma offers an extensive line of attachment and matrix factors. Each lot is cell culture tested to assess its ability to promote cell attachment and spreading.
Collagen
Chondroitin Sulfate A
ECM gel
Fibronectin
Gelatin
Laminin
Superfibronectin
Thrombospondin
Vitronectin
Proteoglycans
Poly-Lysine
Disintegrins
Fibronectin Proteolytic Fragments
Proteoglycans
Proteoglycans, which consist of a core protein with one or more
glycosaminoglycan side chains, are a significant component of the extracellular
matrix. Proteoglycans interact with a variety of molecules in the extracellular matrix,
including various cell adhesion molecules and growth factors. To assist in your extracellular
matrix research, Sigma now offers several proteoglycans isolated from different sources.
Aggrecan
Activity:
Combines with hyaluronic acid to form a very large cartilage macromolecular complex.
A 2 mg/ml solution of aggrecan will increase the relative viscosity of a 4% hyaluronic acid solution by 40%.
| Product Number |
Source |
Storage |
| A 1960 |
Bovine articular cartilage |
-0°C |
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Biglycan
Activity:
Binds to TGF-b; binds to collagen type I in low ionic strength
(less than 3 mM phosphate) buffer. At higher ionic strengths, biglycan does not bind to collagen
type I. It enhances the inhibition effect of TGF-b on osteoclast proliferation
at a concentration of 4-20 µg/ml. It also prevents the attachment of CHO cells to fibronectin,
with a 50% inhibition at 17-21 µg/ml.
| Product Number |
Source |
Storage |
| B 8041 |
Bovine articular cartilage |
-0°C |
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Decorin
Activity:
Binds to TGF-b; binds to collagen type I. It enhances the
inhibition effect of TGF-b on osteoclast proliferation at a
concentration of 4-20 µg/ml. It also prevents the attachment of
CHO cells to fibronectin, with a 50% inhibition at 3-6 µg/ml and 100% inhibition at 10-20 µg/ml.
| Product Number |
Source |
Storage |
| D 8428 |
Bovine articular cartilage |
-0°C |
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Heparan Sulfate Proteoglycan (HSPG) Sterile-filtered solution
Activity:
Binds to a variety of molecules found in the extracellular matrix, including laminin, fibronectin,
collagen type IV, and FGF-basic. It induces high affinity binding of FGF-basic to cells deficient in
heparan sulfate and soluble FGF receptors at a concentration of 10-100 ng/ml.
| Product Number |
Source |
Storage |
| H 4777 |
Basement membrane of Engelbreth-Holm-Swarm mouse sarcoma |
-20°C |
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Poly-Lysine
Sigma offers both Poly-D-Lysine and Poly-L-Lysine in several molecular weight
ranges. Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the
cell membrane and positively-charged surface ions of attachment factors on the culture surface.
When adsorbed to the culture surface, it increases the number of positively charged sites
available for cell binding.
Target cells for attachment:
Attachment of a variety of cell types
Product Use
Poly-D-Lysine Hydrobromide, Lyophilized, Sterilized by g irradiation
| Product Number |
Molecular Weight |
Source |
Storage |
Concentration for Use |
| P 7280 |
MW 30,000-70,000 |
Synthetic |
2-8°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
| P 6407 |
MW 70,000-150,000 |
Synthetic |
2-8°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
| P 7405 |
MW >300,000 |
Synthetic |
2-8°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
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Poly-L-Lysine Hydrobromide, Lyophilized, Sterilized by
g irradiation
| Product Number |
Molecular Weight |
Source |
Storage |
Concentration for Use |
| P 9155 |
MW 30,000-70,000 |
Synthetic |
-0°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
| P 6282 |
MW 70,000-150,000 |
Synthetic |
-0°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
| P 5899 |
MW 300,000 |
Synthetic |
-0°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
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Poly-L-Lysine, 0.01% Solution, Sterile
| Product Number |
Molecular Weight |
Source |
Storage |
Concentration for Use |
| P 4707 |
MW 70,000-150,000 |
Synthetic |
-0°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
| P 4832 |
MW 150,000-300,000 |
Synthetic |
-0°C; store solubilized product at -20°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
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Poly-L-Ornithine Hydrobromide, 0.1 mg/ml Solution
| Product Number |
Molecular Weight |
Source |
Storage |
Concentration for Use |
| P 4957 |
MW 30,000-70,000 |
Synthetic |
2-8°C |
Use 0.5 ml of a 0.10 mg/ml solution to coat 25 cm2 |
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Disintegrins
Disintegrins interfere with cell adhesion to the extracellular matrix, including
adhesion of melanoma cells and fibroblasts to fibronectin. They are also potent inhibitors
of platelet aggregation. To meet your research needs, Sigma offers several disintegrins isolated
from different venom sources. These peptides are of low molecular weight, cysteine-rich, and contain
the arginine-glycine-aspartic acid (RGD) sequence. They are all integrin
b1 and b3 inhibitors.
Echistatin, Purity >95% by SDS-PAGE, Lyophilized, Sterilized by
g irradiation
Activity:
Inhibits bone resorption, platelet aggregation (IC50 = 500 nM), fibronectin binding
(IC50 = 2-3 nM)
| Product Number |
Source |
Storage |
| E 1518 |
Echis carinatus |
-20°C |
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Kistrin, Purity >95% by SDS-PAGE, Lyophilized, Sterilized by
g irradiation
Activity:
Inhibits platelet aggregation (IC50 = 100-150 nM),
fibronectin binding (IC50 = 1-4 nM)
| Product Number |
Source |
Storage |
| K 4755 |
Agkistrondon rhodostoma |
-20°C |
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Fibronectin Proteolytic Fragments
Proteolytic fragments can be used for mapping regions, functions, and activities of fibronectin.
Target Cells:
- Epithelial cells
- Mesenchymal cells
- Neuronal cells
- Fibroblasts
- Neural crest cells
- Endothelial cells
Fibronectin Proteolytic Fragment 30kDA, Heparin binding fragment,
Purity >90% by SDS-PAGE, Lyophilized
| Product Number |
Source |
Storage |
Binding Activity |
| F 9911 |
Human Plasma fibronectin prepared by trypsin digestion of 70kDA fragment |
-20°C |
Collagen, Heparin, Fibrin |
Fibronectin Proteolytic Fragment 45kDA, Gelatin binding fragment,
Purity >90% by SDS-PAGE, Lyophilized
| Product Number |
Source |
Storage |
Binding Activity |
| F 0162 |
Human Plasma fibronectin prepared by trypsin digestion of 70kDA fragment |
-20°C |
Gelatin |
Fibronectin Proteolytic Fragment 70kDA, Heparin and gelatin
binding fragment, Purity >90% by SDS-PAGE, Lyophilized
| Product Number |
Source |
Storage |
Binding Activity |
| F 0287 |
Human Plasma fibronectin prepared by Cathepsin D digestion |
-20°C |
Collagen, Heparin, Gelatin, Fibrin |
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PRODUCT USE
Poly-Lysine (Product Nos.
P 7280,
P 6407,
P 7405,
P 9155,
P 6282,
P 5899,
P 4707*,
P 4832*)
Optimal conditions must be determined for each cell line and
application.
- Add 50 ml of sterile tissue culture grade water to 5 mg of poly-
lysine.
- Aseptically coat culture surface with 1.0 ml/25 cm2 of solution.
Rock gently to ensure even coating of the culture surface.
- After 5 minutes, remove solution by aspiration and thoroughly
rinse surface with sterile tissue culture grade water.
- Allow to dry at least 2 hours before introducing cells andmedium.
*Note: Step 1 is not necessary for Poly-lysine solution, Product
Nos. P 4707 and P 4832.
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