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Stemline™ Platform
Hematopoietic Cytokines
Stemline™ Platform - Media for expansion and maturation of stem cells.
| Prod. # |
Product Name |
Size
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| K3136 |
Keratinocyte Medium Supplement, 100x, BPE-free |
5 ml |
| S0189 |
Stemline™ Hematopoietic Stem Cell Expansion Medium |
500 ml
6 x 500ml
|
| S0192 |
Stemline™ II Hematopoietic Stem Cell Expansion Medium |
500 ml
6 x 500ml
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| S9945 |
Stemline™ Keratinocyte Growth Supplement |
1 vl |
| S0196 |
Stemline™ Keratinocyte Medium II |
500 ml |
| S3194 |
Stemline™ Neural Stem Cell Expansion Medium |
500 ml
|
| S1694 |
Stemline™ T Cell Expansion Medium |
1 L
|
The use of human stem cells (HSC) isolated from umbilical cord blood, peripheral blood, or bone marrow as a source of cellular reconstitution following high-dose chemotherapy is now a common therapeutic modality for the treatment of malignancy. However, due to the low yield of HSC from typical sources, HSC transfusion has historically been most effective in children with limited applications and marginal success in adults. In recent years the development of media used to expand and mature adult stem cells has greatly increased the number of candidates eligible as well as the success rate of adult stem cell therapy. In order to obtain sufficient numbers of cells for applications of this therapeutic approach in adults, ex vivo expansion has been utilized to ensure successful engraftment and minimize the short-term effects of neutropenia and thrombocytopenia. To this end, Sigma-Aldrich has developed the Stemline™ Platform that includes media optimized for the expansion and maturation of various adult stem cell types.
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Cell Expansion and Consistency:
A Foundation for Stem Cell Research |
Stemline HSC Expansion Medium exceeds two leading competitors as determined by Total Nucleated Cell (TNC) counts when tested in 24-well tissue culture plates over 14 days. From day 7 to day 14, the TNC count in Stemline was markedly greater.
Cells were seeded in triplicate at 10,000 cells per well in 24-well tissue culture plates containing either Stemline Hematopoietic Stem Cell Expansion Medium or 2 leading competitors. The wells were supplemented with 100 ng/ml each of SCF, G-CSF, and TPO. Each well was triturated and harvested after either a 7-day or 14-day expansion. Total nucleated cells were counted using a CASY-1 cell counter (Schärfe Systems) and averaged for all 3 wells in each condition. |
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Stemline also performs better than the competition in Teflon® culture bags. Stemline produces higher TNC counts after 14 days. High TNC counts are an important factor in laying the foundation for using these cells in therapeutic applications.
Cells were seeded in Teflon culture bags containing either Stemline Hematopoietic Stem Cell Expansion Medium or 2 leading competitors. The wells were supplemented with 100 ng/ml each of SCF, G-CSF, and TPO. The cells were expanded for 7 days prior to being harvested and seeding a new culture bag for an additional 7 days. Cells were harvested from the second culture bag and total nucleated cells were counted. |
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Press Release: Sigma-Aldrich Submits Device Master File for Its Stemline™ Medium to the Food & Drug Administration
Feature Article: Serum-Free Expansion of CD34+ Umbilical Cord Blood Using Stemline™ Hematopoietic Stem Cell Expansion Medium
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The following table lists a group of cytokines whose predominant actions involve the stimulation or regulation of hematopoietic cells.
The term "colony stimulating factor" (CSF) was originally given to agents recognized to stimulate the growth of colonies containing differentiated myeloid cells from a single bone marrow-derived precursor cell plated in semi-solid agar. Glycoproteins considered to be CSFs include: granulocyte colony-stimulating factor (G-CSF); granulocyte-macrophage colony-stimulating factor (GM-CSF); macrophage colony-stimulating factor (M-CSF); interleukin-3 (multi-CSF or IL-3); interleukin-5 (IL-5); erythropoietin (EPO), and thrombopoietin (TPO).
The formation and maturation of hematopoietic cells are also affected by cytokines of the structural class know as the "4-alpha-helical bundle" family. These include stem cell factor (SCF) and flt-3/flk-2 Ligand (FL).
These growth factors and cytokines are intended for research use only and are not intended for use in clinical procedures.
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| Prod. # |
Description |
Size |
Stimulated Cells |
|
E5627
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Erythropoietin (EPO), Human, recombinant |
10 units
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Erythroid progenitor cells. EPO receptors are lost during cell differentiation. |
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F3422
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FLT-3/FLK-2 Ligand, Human, recombinant |
5 µg
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Primitive hematopoietic (multi-lineage) progenitor cells that express the flt3 receptor.
Myeloid and pro-B cells of mouse, rat, cat, rabbit, primate and human origin. |
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G0407
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Granulocyte Colony-Stimulating Factor (G-CSF), Human, recombinant |
2 µg
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Proliferation, differentiation, survival and activation factor for hematopoietic restricted granulocyte lineage cells.
Neutrophils, myeloid leukemic cells, neutrophilic granulocytes from bone marrow and placental trophoblasts.
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G8160
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Granulocyte Colony-Stimulating Factor (G-CSF), Mouse, recombinant |
5 µg
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Proliferation, differentiation, survival and activation factor for hematopoietic restricted granulocyte lineage cells.
Neutrophils, myeloid leukemic cells, neutrophilic granulocytes from bone marrow and placental trophoblasts. |
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G5035
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Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Human, recombinant |
5 µg
|
Growth, differentiation, and essential survival factor for granulocyte, macrophage and eosinophil lineage cells from progenitor stage to maturity.
Receptors on most types of myeloid progenitor cells, mature monocytes, neutrophils, eosinophils, basiophils, and dendritic cells.
Stimulates some epithelial cells and osteoclasts. |
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G0282
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Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Mouse, recombinant |
5 µg
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Growth, differentiation, and essential survival factor for granulocyte, macrophage and eosinophil lineage cells from progenitor stage to maturity.
Receptors on most types of myeloid progenitor cells, mature monocytes, neutrophils, eosinophils, basiophils, and dendritic cells.
Stimulates some epithelial cells and osteoclasts. |
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I1646
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Interleukin-3 (IL-3), Human, recombinant |
10 µg
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Multifunctional cytokine.
Supports early development of multi-potential hematopoietic progenitor cells: macrophage, neutrophils, mast cells and megakaryocytes from bone marrow.
Stimulate T-cells and induce IgG secretion from activated B-cells. |
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I4144
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Interleukin-3 (IL-3), Mouse, recombinant |
10 µg
|
Multifunctional cytokine.
Supports early development of multi-potential hematopoietic progenitor cells: macrophage, neutrophils, mast cells and megakaryocytes from bone marrow.
Stimulate T-cells and induce IgG secretion from activated B-cells. |
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I1395
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Interleukin-6 (IL-6), Human, recombinant |
10 µg
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Multifunctional cytokine which acts on a wide range of cell types.
In combination with other cytokines, it acts upon; fibroblasts, myeloid progenitor cells, T-cells, B-cells, and hepatocytes. |
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I9646
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Interleukin-6 (IL-6), Mouse, recombinant |
5 µg
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Multifunctional cytokine which acts on a wide range of cell types.
In combination with other cytokines, it acts upon; fibroblasts, myeloid progenitor cells, T-cells, B-cells, and hepatocytes. |
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M6518
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Macrophage Colony-Stimulating Factor (M-CSF), Human |
5 µg
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Primarily targets macrophages and stimulates multiple responses, including; proliferation, cytokine and inflammatory modulator release, cytotoxicity and pinocytosis.
Also, supports osteoclast differentiation and may act on placental trophoblasts. |
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M9170
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Macrophage Colony-Stimulating Factor (M-CSF), Mouse |
5 µg
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Primarily targets macrophages and stimulates multiple responses, including; proliferation, cytokine and inflammatory modulator release, cytotoxicity and pinocytosis.
Also, supports osteoclast differentiation and may act on placental trophoblasts. |
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S7901
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Stem Cell Factor (SCF), Human, recombinant |
10 µg
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Broad activities involving hematopoietic, pigment and primordial germ cell lineages.
A potent synergistic co-stimulating growth factor which increase the size, but not the differential, of myeloid, erythroid, and lymphoid lineage colonies. |
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T4309
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Thrombopoietin (TPO), Human, recombinant |
5 µg
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Primary regulatory factor for megakaryocytopoiesis and thrombopoiesis.
Regulates the growth and maturation of megakaryocytes and their progenitors. |
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T4184
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Thrombopoietin (TPO), Mouse, recombinant |
5 µg
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Primary regulatory factor for megakaryocytopoiesis and thrombopoiesis.
Regulates the growth and maturation of megakaryocytes and their progenitors. |
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