Monoclonal Anti γ Parvin

Monoclonal Anti-γ Parvin: Focal adhesion protein

Prod. No. P5746
Clone γ parvin 40, developed in mouse
Purified mouse immunoglobulin in phosphate-buffered saline
Immunogen: peptide corresponding to the C-terminal region of human γ parvin (amino acids 314-328)
Isotype: mouse IgG1
Species Reactivity: The antibody recognizes human, monkey and bovine γ parvin; it does not react with mouse or rat γ parvin

Cell adhesion to the extracellular matrix (ECM) is an important process that controls cell morphology, proliferation, migration, differentiation and survival. The engagement of integrin molecules on the cell surface during cell adhesion to the ECM is accompanied by recruitment of multiple cytoskeletal and signaling proteins to focal adhesion sites [1-4]. These proteins link the cytoskeleton to the ECM and mediate signal transduction between the ECM and the intra-cellular compartment.

The parvin protein family belongs to the focal adhesion proteins that comprise three members referred to as α, β, and γ parvin [5]. α Parvin is ubiquitously expressed, β parvin is expressed in heart and skeletal muscle, and γ parvin is expressed in lymphoid tissues. Development studies have shown that α parvin is expressed throughout mouse development, while β parvin is gradually up-regulated and γ parvin is down-regulated at day 11. In cancer cells, the levels of the three parvins are down-regulated compared to normal tissues [5].

Immunoblot using a total extract from 293T cells detected a band at 42 kDa. Monoclonal antibodies specific for γ parvin are an important tool for studying the biology of focal adhesion proteins.

Applications: Immunoblotting, Indirect ELISA

1. Turner, C.E. and Burridge, K., Transmembrane molecular assemblies in cellextracellular matrix interations., Curr. Opin. Cell Biol., 3, 849-853 (1991).
2. Turner, C.E., Paxillin and focal adhesion signaling., Nature Cell Biol., 2, E231-E236 (2000).
3. Turner, C.E., Paxillin interactions., J. Cell Sci., 113, 4139-4140 (2000).
4. Giancotti, F.G. and Rouslahti, E., Integrin signaling., Science, 285, 1028-1032 (1999).
5. Korenbaum, E., et al., Genomic organization and expression profile of the parvin family of focal adhesion protens in mice and humans., Gene, 279, 69-79 (2001).

Related Products