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The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in a partnership with Sigma-Aldrich. A list of products is available to use in each protocol.
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Selected Protocol from ""Live Cell Imaging",
Frank Alexis, Jieming Zeng, and Wang Shu
PEI Nanoparticles for Targeted Gene Delivery
Frank Alexis, Jieming Zeng, and Wang Shu
ABSTRACT
This protocol describes the preparation of polyethylenimine (PEI)/DNA nanoparticles for targeted gene delivery. This delivery strategy improves the efficiency of gene transfer by enhancing the entry of gene vectors into the desired cells and reducing uptake by nontarget cells. We describe here methods for the conjugation of targeting peptides to PEIs, formation of DNA complexes using the conjugated PEIs or nonconjugated PEIs together with targeting peptides, and cell transfection using these complexes. The conjugation step involves the use of the succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), a heterobifunctional cross-linker, to form a stable bond between PEI and peptides containing thiol groups.
Products Available for this Protocol
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Buffers,
Solutions, and Reagents |
|
|
|
| Lithium chloride |
L9650 |
Lithium chloride, for molecular biology |
|
| DMSO |
D8418 |
Dimethyl sulfoxide, for molecular biology |
|
| PEI polymers |
03880 |
Ethylene imine polymer solution |
|
| Phosphate buffered saline (PBS) |
P5368 |
Phosphate buffered saline, pH 7.4, Biotechnology Performance
Certified |
|
| Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate |
M5525 |
4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide
ester |
|
| Water, ultrapure sterilized |
W4502 |
Water, Molecular Biology Reagent |
|
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