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Sigma® combines speed and convenience with performance in the Imprint™ Chromatin Immunoprecipitation Kit (CHP1). The Imprint ChIP Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2). The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.
- Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
- Sensitive—As few as 20,000 cells required for each ChIP sample
- Convenient—Fewest steps of any available ChIP protocol
- Flexible—Protocols for cells or tissue, and convenient
strip-well format for high-throughput applications
- Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology
View the poster Rapid and easy method for Chromatin IP (ChIP) analysis of metastatic cells
(446 KB PDF).
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Figure 1. Comparison of time required for protocol completion from fixation through purification using different Chromatin Immunoprecipitation kits. Each ChIP experiment was carried out using each manufacturers recommended protocol and total required times were compared. |
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Figures 2 and 3. A highly sensitive ChIP assay. HeLa cells were counted, fixed, and immunoprecipitated according to the Imprint CHP1 bulletin with optional High Sensitivity method. ChIP assays were performed using anti-H3K9ac (H9286) and kit provided antibodies against: human RNA Polymerase II and non-specific mouse IgG. To evaluate ChIP enrichment, SYBR qPCR was conducted targeting the GAPDH promoter region (highly expressed house-keeping gene). Percent Input describes the amount of DNA with and without antibody selection. For these antibodies, apparent enrichment increased with fewer cells per reaction from diminished non-specific pull-down.
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