Sigma-Aldrich® has entered an exclusive partnership with Rosetta Inpharmatics, a leader in advanced siRNA research, to use the proprietary Rosetta siRNA Design Algorithm to design its MISSION line of siRNA. The Rosetta siRNA Design Algorithm utilizes Position-Specific Scoring Matrices (PSSM) and knowledge of the all-important siRNA seed region to predict the most effective and specific siRNA sequences for your target gene of interest. Additionally, the Rosetta siRNA Design Algorithm was developed utilizing data from over three years of gene-silencing experiments, ensuring that the algorithm’s in-silico rules are guided and bolstered by real-world empirical evidence.
Sigma-Aldrich guarantees that two out of three siRNA duplexes per target gene will achieve knockdown efficiencies of greater than or equal to 75%.
Benefits of pre-designed MISSION siRNA, powered by the Rosetta siRNA Design Algorithm:
- Best-in-class, guaranteed gene silencing
- Efficient knockdown of low abundance messages
- siRNAs targeting every gene in the current human, mouse, and rat genomes
- Simplify transfection optimization with 11 MISSION Positive Control siRNA
- Over 700 Mission pre-designed siRNA have been functionally validated
- Individual Mission pre-designed siRNA or Pre-arrayed siRNA Libraries
Ordering MISSION siRNA for Single Gene Targets
We've made ordering MISSION siRNA for single gene targets easy through Sigma's state-of-the-art web interface,
Your Favorite Gene.
To find a MISSION siRNA that knocks down your gene, simply search on your gene of interest by gene name, symbol, RefSeq, or Gene ID number.
|
MISSION Pre-designed siRNA provide best-in-class gene silencing.
Click on chart for larger image. |
|
Hela cells transfected at a concentrate of 30 nM siRNA. Remaining gene expression levels measured via quantitative real-time PCR 48 hours after transfection (relative to mock). Data represents the mean of four biological replicates.
Lower Score = Increased Gene Silencing |
back to top
Selected Citations—MISSION Pre-Designed siRNA and Pre-arrayed siRNA Libraries
Marine, S. et al. Activity profile-based siRNA screen to explore the functional genomics of Alzheimer’s disease. BioTechniques Dec 2007, 43, Supplement, Large-Scale Genomic Analysis: S22–S27.
Espeseth, A. S. et al. Genome wide analysis of ubiquitin ligases in APP processing identifies a novel regulator of BACE1 mRNA levels. Mol. Cell. Neurosci. Nov 2006, 33(3), 227–35.
Bartz, S. R. et al. Small interfering RNA screens reveal enhanced cisplatin cytotoxicity in tumor cells having both BRCA network and TP53 disruptions. Mol. Cell. Biol. Dec 2006, 26(24), 9377–86.
Majercak, J. et al.LRRTM3 promotes processing of amyloid-precursor protein by BACE1 and is a positional candidate gene for late-onset Alzheimer’s disease. Proc. Natl. Acad. Sci. U.S.A. Nov 2006, 103(47) 17967–72.
Pre-arrayed MISSION siRNA Libraries Ordering Inforamtion
MISSION siRNA Human Druggable Genome
MISSION siRNA Rat Druggable Genome
MISSION siRNA Mouse Kinase Library
Send this page to a colleague.
Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.
back to top
RNAi Homepage
|
)