Learn from the Experts in RNAi! Are you interested in learning more about vector-based RNAi? Do you find viral packaging & delivery concepts challenging? Would hands-on experience with siRNA and shRNA be helpful? Look no further! Sigma is providing an educational workshop just for you.
Workshop: Synthetic and Viral-based Systems for Gene Silencing Date: April 2-4, 2008 Where: Sigma Life Science and High Technology Center St. Louis, Missouri Map Hurry! Registration ends March 7th.	Register Now for a laboratory and seminar-based workshop on RNAi!

Overview | Workshop | Accommodations | Register Now!

Overview
RNAi has emerged as an important mainstream tool for both basic and applied research, and it has revolutionized the novel discipline known as functional genomics. This workshop will provide hands-on technical training for both siRNA and lentiviral shRNA, experimental design, troubleshooting, and measuring knockdown effects. Featured lectures will provide in depth discussion on the fundamentals of RNAi as well as cutting edge applications for drug discovery and target validation. Registrants should be familiar with basic mammalian cell culture techniques.

Workshop– Synthetic and Viral-based Systems for Gene Silencing

Lecture and Laboratory Topics
RNAi mechanism
Applications for RNAi
siRNA and shRNA methodologies
Detection methods
RNAi in difficult cell types
Vector based shRNA
Viral delivery systems
High-throughput screening
Packaging methods
Titer methods
Optimizing MOI
Stable vs. transient assays
Proper controls
Viral handling
Transduction optimization
mRNA quantitation methods
Troubleshooting discussion

Register Now!

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For additional details, email MissionRNAi@sial.com

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