Learn from the Experts in RNAi! Are you interested in learning more about vector-based RNAi? Do you find viral packaging & delivery concepts challenging? Would hands-on experience with siRNA and shRNA be helpful? Look no further! Sigma is providing an educational workshop just for you.
Workshop: Synthetic and Viral-based Systems for Gene Silencing Date: October 28-30, 2008 Where: Sigma Life Science and High Technology Center St. Louis, Missouri Map Hurry! Registration ends October 7, 2008.	Register Now for a laboratory and seminar-based workshop on RNAi!

Overview | Workshop | Accommodations | Register Now!

Overview
RNAi has emerged as an important mainstream tool for both basic and applied research, and it has revolutionized the novel discipline known as functional genomics. This workshop will provide hands-on technical training for both siRNA and lentiviral shRNA, experimental design, troubleshooting, and measuring knockdown effects. Featured lectures will provide in depth discussion on the fundamentals of RNAi as well as cutting edge applications for drug discovery and target validation. Registrants should be familiar with basic mammalian cell culture techniques.

Workshop– Synthetic and Viral-based Systems for Gene Silencing

Current Speakers
Hakim Djaballah, Ph.D., Director, HTS Core Facility at MSKCC

Past Speakers
Rick Cohen, Ph.D., Director of hESC Core Facility at Rutgers
Aubrey Thompson, Ph.D. Professor of Biochemistry and Molecular Biology at Mayo Clinic
Richard Hotchkiss, M.D. Professor of Anesthesiology, Medicine and Surgery at Washington University
Michael McManus, Ph.D. Assistant Professor at UCSF Diabetes Center
Sheila Stewart, Ph.D. Assistant Professor Cell Biology and Physiology at Washington University

Testimonials from prior participants:

“Absolutely awesome. Everyone at Sigma is very receptive and ready to assist with all needs. I would highly recommend this workshop to anyone in the field. I am very glad that I had this opportunity.” - Laia Gómez Baldó, Institute Català d'Oncologia, Spain

“The Sigma-Aldrich RNAi workshop included a nice balance of theoretical and practical information. In particular, the hands-on components of the workshop showed me how easily I could incorporate siRNA and shRNA approaches into my research projects. The Sigma-Aldrich RNAi team was knowledgeable, helpful, and above all, friendly and accessible.” - Jon Fisher, St. Louis University Biology Department, Missouri, USA

"I just wanted to thank you for the hospitality you have shown us during our stay in St. Louis. It was a very pleasant experience and it felt very good to know that there are people that not only care about the science but also about fellow human beings. The workshop was very nicely arranged, if there were any hiccups, I did not see any. The crew was also very helpful and friendly, that counts a lot for me..." - Phiyani Lebea, at CSIR Biosciences, South Africa

Lecture and Laboratory Topics
RNAi mechanism
Applications for RNAi
siRNA and shRNA methodologies
Detection methods
RNAi in difficult cell types
Vector based shRNA
Viral delivery systems
High-throughput screening
Packaging methods
Titer methods
Optimizing MOI
Stable vs. transient assays
Proper controls
Viral handling
Transduction optimization
mRNA quantitation methods
Troubleshooting discussion

Register Now!

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For additional details, email MissionRNAi@sial.com

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