MISSION® Protocols

Cold Spring Harbor Laboratory Press Protocols

• Culturing Trophoblast Stem (ST) Cell Lines
• In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
• Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for
  Specific and Effective Gene Silencing in Mammalian Cells
• Production of dsRNA for RNAi in Avian Embryos
• Microinjection of dsRNA into Mouse Oocytes and Early Embryos
• Genetic Manipulation of Mammalian Cells by Microinjection
• Mammalian RNA Interference, Thomas Tuschl Protocols
• Lentivirus Transduction of Hematopoietic Cells Protocol
• Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors

Lentiviral Transduction Protocol (69.0 Kb PDF)

Lentiviral Transduction Protocol without puromycin

Lentiviral Titer by Limiting Dilution Protocol (63.6 Kb PDF)

Lentiviral Transduction of Suspension Cells—Spinoculation Protocol

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