TargeTron® Gene Knockout System

Introduction to TargeTron The TargeTron Gene Knockout System (TA0100) is a revolutionary method for rapid and specific disruption of genes in prokaryotic organisms. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches.

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Features

• Targeted and permanent gene disruption
• Simple, streamlined protocol; Knockouts in 3 days or less
• Minimal screening to isolate mutants
• >90% successful targeted insertion
• No specific host factor requirements
• Pre-screen your target sequence on the TargeTron Test Site

Resources
TargeTron Vectors
Detailed Description and Algorithm
TargeTron Test Site
TargeTron Design Site
User Guide (1.1 MB PDF)
pACD4K-C Vector Sequence (7.7 KB TXT)
Updated License Agreement (86 KB PDF)
Poster (1.6 MB PDF)
Frequently Asked Questions
References
Ordering Information

Host Range
TargeTron has been validated in multiple organisms; however, work to adapt the system to other organisms is ongoing and data will be provided as it becomes available.

Organism	Reference
Agrobacterium tumefaciens	Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector
Bacillus subtilis (TargeTron Vector pNL9164, Product No. T6701)	—
Clostridium acetobutylicum	The ClosTron: A universal gene knock-out system for the genus Clostridium
Clostridium botulinum	The ClosTron: A universal gene knock-out system for the genus Clostridium
Clostridium difficile	The ClosTron: A universal gene knock-out system for the genus Clostridium
Clostridium perfringens (TargeTron Vector pJIR750ai, Product No. T7701)	Disruption of a toxin gene by introduction of a foreign gene into the chromosome of Clostridium perfringens using targetron-induced mutagenesis
Clostridium sporogenes	The ClosTron: A universal gene knock-out system for the genus Clostridium
Escherichia coli (TargeTron Gene Knockout System, Product No. TA0100)	—
Lactococcus lactis (TargeTron Gene Knockout System, Product No. TA0100)	—
Proteus mirabilis	Reference in press
Pseudomonas aeruginosa	Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector
Salmonella typhimurium (TargeTron Gene Knockout System, Product No. TA0100)	—
Serratia marcescens	Unpublished to date
Shigella flexneri (TargeTron Gene Knockout System, Product No. TA0100)	—
Staphylococcus aureus (TargeTron Vector pNL9164, Product No. T6701)	—
Xylella fastidiosa	Unpublished to date

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Related Products
Below is an easy guide to materials needed for or that complement the TargeTron Gene Knockout System.

• TargeTron Vectors
• GenElute™ PCR Clean-up Kit (NA1020)
• Quick Link™ DNA Ligation Kit (LIG-2)
• Water, Molecular Biology Reagent (W4502)
• GC5™ Competent Cells (G3169)
• BL21 (DE3)-T1^R Competent Cells (B2935)
• SOC Medium (S1797)
• LB Broth, liquid (L2542)
• LB Agar, EZ Mix™ Powder (L7533)
• LB agar plates
• IPTG (I6758)
• X-gal (B9146)
• Pre-cast Agarose Gels

• siRNA
• MISSION^® shRNA

Sigma-Genosys

This product and its use are the subject of one or more of U.S. Patent Nos. 5,698,421, 5,804,418, 5,869,634, 6,027,895, 6,001,608, and 6,306,596 and/or other pending U.S. and foreign patent applications controlled by InGex, LLC.
TargeTron is a registered trademark of InGex, LLC.

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