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The TargeTron Vector pNL9164 (T6701) is a circularized, expression vector derived from pNL91621. The vector can be used for targeted gene knockouts in gram-positive organisms such as Staphylococcus aureus1. As supplied, the pNL9164 vector is retargeted to the hsa gene in S. aureus. For validation in S. aureus, this vector can be used directly to knockout hsa without any further modifications (protocol included). In order to retarget this vector to other genes, please refer to pages 6-9 in the TA0100 User Guide (1.1 MB PDF).
TargeTron is a registered trademark of InGex, LLC.
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TargeTron® Gene Knockout System
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