MISSION™ TRC shRNA Library

Sigma Brings You the Best of TRC-1, with TRC-2 Now Underway!

Over 700 shRNA gene targets have already been validated, search Your Favorite Gene Search to locate shRNAs that target your gene. Look for the validation symbol located on the shRNA detail pages to identify target sets that contain 1 or more functionally validated shRNAs.

Introduction to TRC and the MISSION shRNA Libraries
The RNAi Consortium (TRC) is a collaborative group of world-renowned academic and corporate life science research groups whose “mission” is to create comprehensive tools for functional genomics research and make them broadly available to scientists worldwide. Sigma-Aldrich is both a collaborative member and supplier of TRC shRNA libraries (MISSION TRC libraries). The TRC-1 human and mouse shRNA libraries were developed at the Broad Institute of MIT and Harvard and currently consist of 159,000 pre-cloned shRNA constructs targeting 16,000 annotated human genes (MISSION TRC-Hs 1.0) and 15,950 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated gene silencing. Sigma is the exclusive manufacturer of TRC-1 clones in DNA and lentiviral formats. Glycerol format products are also available.

Sigma-Aldrich is also a member and distributor of TRC-2. Current plans for TRC-2 include functional testing and validation of the TRC-1 library, creation of an additional 150,000 clones (total of 300,000), and functional testing of the new TRC-2 clones, while looking at improved methods for screening. Combined efforts of The Broad Institute and Sigma-Aldrich have already generated validation data for over 700 gene targets that are now designated as “Validated” on the shRNA detail pages (accessed through the shRNA and YFG search tools). Validation requires that 1 or more shRNAs have been functionally tested and have demonstrated 70% or greater knockdown levels.

Sigma-Aldrich is committed to providing unmatched infrastructure and service for RNAi. With the addition of state-of-the-art robotics, liquid handling, LIMS, and dedicated laboratories in our new Life Science & High Technology Center, our goal is to provide superior quality and convenient service for your RNAi research needs.

Search the MISSION Target Search database  
Locate shRNA sets specific for your gene of interest. The database may be searched using RefSeq or Accession Numbers, gene symbols, gene descriptions among other specific identifiers.

Comprehensive shRNA Collection 159,000 pre-cloned shRNA constructs Targets 16,000 human and 15,950 mouse genes TRC-2 functional validation of TRC-1 and library expansion is now underway	High Quality Manufacturing System and Personnel HT robotic handling 1D and 2D barcodes Full staff of R&D, production, QC & QA professionals
Optimal Lentiviral-Based RNAi Stable or transient transfection Viral particles for transduction Integrates for long term knockdown Transduces virtually any cell type (dividing and non-dividing) No interferon response Lack of recombination issues Robust and consistent viral titers	Leader in Lentiviral shRNA Products and Support Extensive web-based resources Technical and R&D experts on staff   Proven System for High Throughput (HT) HT viral production High content screening
shRNA Design for High Performance 2 of 5 clones typically provide 70% or greater knockdown levels. Sequence, specificity & position scoring with Broad Institute algorithm Comprehensive target coverage with ~5 clones per gene 3’ UTR shRNA constructs for phenotypic rescue studies	Wide Array of Product Formats Libraries Gene family sets Target sets and single clones Glycerol, DNA & viral formats

shRNA Design
MISSION shRNA clones, designed and developed by TRC at the Broad Institute of MIT and Harvard, include hairpin sequences comprised of a 21 base stem and a 6 base loop. Rules based design consisting of sequence, specificity, and position scoring are utilized to generate optimal shRNA sequences. The hairpin sequences are each cloned into the pLKO.1 vector² and sequence verified. Typically, 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript. Two in five clones will typically provide at least 70% knockdown of the gene target. Numerous target sets include a shRNA clone targeting the 3'UTR for use in phenotypic rescue studies using cDNA expression constructs.

Gene Silencing Using MISSION shRNA Click here to view enlarged image (259 Kb PDF)

Figure 1. High-throughput validation of MISSION-TRC shRNA lentiviral transduction particles. Gene silencing effect of 60 different genes was measured at the mRNA transcript level and compared to pLKO.1-puro empty control particles. For each target gene, approximately 70% or greater knockdown was achieved.

Citing the Library in Publications
In scientific publications, the human library should be referred to as MISSION™ TRC-Hs 1.0 (Human) while the mouse library should be referred to as MISSION™ TRC-Mm 1.0 (Mouse). Individual clones should be identified by their unique TRC number (e.g. MISSION™ shRNA TRCN0000030720).

References

1. Moffat J. et al., A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell, 124, 1283-1298. 2006.
2. Stewart, S.A., et al., Lentivirus-delivered stable gene silencing by RNAi in primary cells., RNA, 9, 493-501 (2003).
3. Zufferey R, et al., Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo., Nat. Biotechnol. 15, 871-85 (1997).
4. Zufferey R, et al., Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery., J Virol., 72, 9873-80 (1998).

Send this page to a colleague.

Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.

back to top
shRNA Homepage
RNAi Homepage