The TRC collection, developed at the Broad Institute of MIT and Harvard, currently consists of 159,000 pre-cloned shRNA constructs targeting 16,000 annotated human genes (MISSION TRC-Hs 1.0) and 15,950 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated gene silencing. In addition Sigma-Aldrich offers a variety of products to support the downstream validation of your shRNA results. These products include siRNA, antibodies, small molecules, purified peptides and proteins. To specifically see what products we have for your gene of interest utilize the YFG search engine.
| Number of Supporting Products |
|
Human |
Mouse |
| siRNAs |
22128 |
24716 |
| Antibodies |
444 |
83 |
| Small Molecules |
1649 |
1072 |
| Peptides and Proteins |
594 |
70 |
Current Library by Gene Family or Functional Class
Note: This is not an exhaustive list of all gene families or pathways that are represented in the library. It is provided to indicate the scope of the RNAi library.
Gene Family/
Functional Class |
| Amidase |
| Apoptosis |
| Carboxylase |
| Cyclase |
| Cytokine and Chemokine Receptors |
| Cytokines and Chemokines |
| DNA Binding |
| DNA Repair |
| Deacetylase |
| Dehydrogenase |
| Electron Transport |
| Esterase |
| G Protein |
| GPCR |
| GTPase |
| Glucosidase |
| Homeobox |
| Hydrolase |
| Ion Channel |
| Isomerase |
|
|
Gene Family/
Functional Class |
| Kinase |
| Ligase |
| Lipase |
| Methylase |
| Mutase |
| Nuclear Hormone Receptor |
| Nuclease |
| Oxidase |
| Oxidoreductase |
| Phosphatase |
| Polymerase |
| Protease |
| Protein Transport |
| Reductase |
| Spliceosome |
| Synthase |
| Transcription Factor |
| Transferase |
| Tumor Suppressor |
| Tyrosine Kinase |
|
Note: Sigma-Aldrich sells a variety of pre-arrayed gene family sets. The details of these sets can be found on the Gene Families page.
References
-
Stewart, S. A. et al. Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA 2003, 9, 493-501.
-
Zufferey R. et al. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat. Biotechnol. 1997, 15, 871-85.
-
Zufferey R, et al. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J Virol. 1998, 72, 9873-80.
-
Moffat J, et al. A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell 2006, 124, 1283-1298.
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Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.
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