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MISSION shRNA Product Overview
The library is currently provided as lentiviral transduction particles, purified plasmid DNA and frozen bacterial glycerol stock format harboring sequence-verified shRNA. Each gene is represented by a target set that consists of an average of 3-5 individual constructs or clones targeting different regions of the gene sequence. Bacterial cultures may be amplified from the glycerol stocks for use in purification of the shRNA plasmid DNA. Alternatively, the purified plasmid format does not require these extra steps as the growth and DNA purification are completed for you. Target cell lines may be transfected with the purified plasmid for transient or stable gene silencing (puromycin selection).
In addition, self-inactivating replication incompetent lentiviral particles can be produced in packaging cells (HEK 293T) by co-transfection with compatible packaging plasmids. Orders of 5 or less target sets (< 25 constructs) are provided in individual 1.4 ml TrakMates® tubes. Each TrakMates® tube contains a unique 2 dimensional barcode on the bottom of the tube that can be read using a corresponding reader. A printed value corresponding to The RNAi Consortium (TRC) clone number is also provided on each tube. Orders of >5 clone sets (>25 constructs) are automatically provided in a 96-well plate with a one dimensional barcode label on the plate and are provided with a CD containing plate map positions.
Manufacturing and Shipping Timelines
Each target set or individual clone is produced on demand in our state-of-the-art manufacturing facility. The following times are required to produce each format after the order appears in our LIMS manufacturing database:
| SHGLY – Bacterial Stock | | 48 hours |
| SHDNA – Purified DNA | | 2 weeks |
| SHVRS – Lentiviral Transduction Particles | | 4 weeks |
Orders ship Monday -- Thursday. Glycerol format orders received after 3 pm on Wednesday may not ship until the following Monday. Some orders may require additional time to process. Please allow up to 2 weeks additional shipping time for international orders.
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Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.
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