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ExpressMag Delivery—Magnify Your Results!
Magnetic beads that further improve lentiviral-based delivery to non-transfectable cells.
- Increase transduction efficiencies up to 10 fold or more
- Accelerate the transduction process by an entire day
- Universal solution for cell lines, primary cells, and suspension cultures
- Synchronize and target viral delivery
- Concentrate the viral dose onto cells for maximum performance
- Validated for use with MISSION® shRNA lentiviral particles
Figure 1. Suspension HEK 293T cells were transduced with MISSION® TurboGFP™
control lentiviral particles (Product No. SHC003V). Cells were transduced with a MOI of 1
and incubated for 49 hours. A) Traditional transduction with Polybrene. B) Cells transduced
using ExpressMag system.
Go Directly to:
Overview of the ExpressMag System
How does ExpressMag Delivery Fit into Your shRNA Project?
ExpressMag Product Information
High-throughput Assay Optimization Overview
Primary Cells Successfully Transduced with ExpressMag Reagents
Contact Us
Overview of the ExpressMag System
Although the ExpressMag system is designed to work with a variety of viruses, it has been specifically validated to support the MISSION® lentiviral shRNA products. Lentiviral particles are known to improve delivery of genetic information to target cells compared to plasmid-based transfection. However, many cell types are still considered difficult to transduce with lentiviral particles (primary cells, suspension cells, etc.). ExpressMag combines the use of magnetic beads with lentiviral particles to further improve delivery to recalcitrant cells.
When ExpressMag is mixed with MISSION® lentiviral particles, the particles bind to the proprietary polymeric shell. This mixture is added into the tissue culture media already on the cells. No media change is required. The tissue culture plate or flask is then placed over an ExpressMag magnetic plate for 15 minutes. During this step, the magnetic nanoparticles are rapidly pulled into contact with the cell surface. The increase in the local concentration of viral particles significantly increases the transduction efficiency. Culture plates are then returned to the incubator, and your experiment proceeds according to your standard protocol. The only difference is that your transduction efficiency is rapidly increased and your results have been magnified.
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How does ExpressMag Delivery Fit into Your shRNA Project?
ExpressMag delivery should be introduced into your project at the optimization stage of your transduction. After the appropriate cell line has been selected for your project, determine whether the cells are easily susceptible to lentiviral delivery (recommended to use the MISSION® TurboGFP™ control lentiviral particles, Product No. SHC003V). If the cell line is difficult to transduce using standard methods, consider introducing ExpressMag reagents into your experiment. ExpressMag beads may be used with MISSION® lentiviral particles at standard titer (1 × 106 TU/ml) or can be combined with high titer virus obtained through our custom large-scale offering.
Utilizing your protocol that is currently working, simply add ExpressMag in at the transduction step. Start with your current viral titer in one well and perform order of two fold reductions in viral load down to a MOI (Multiplicity of Infection) of 1. While ExpressMag has been demonstrated to be non-toxic in a variety of cell lines, always include a negative control virus (Product No. SHC002V) and ExpressMag beads.
ExpressMag Product Information
There are two magnetic plates available in the ExpressMag line. The ExpressMag Super Magnetic Plate (Product No. SHM04) can be used in conjunction with all standard multi-well plates, tissue culture dishes up to 5 cm, and tissue culture flasks up to T-75. The ExpressMag 96-Well Magnetic Plate (Product No. SHM05) has been optimized for assays in 96-well plate format.
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| Product No. |
Package Size |
Description |
Number of Transductions |
| 96-well plate |
24-well plate |
T-25 |
| SHM01 |
1 KT |
MISSION® ExpressMag Super Magnetic Kit
Includes 1 Super Magnetic Plate and 100 µl of ExpressMag Beads |
63 |
15 |
1 |
| SHM02 |
1 KT |
MISSION® ExpressMag 96-Well Magnetic Kit
Includes 1 96-Well Magnetic Plate and 100 µl of ExpressMag Beads |
63 |
15 |
1 |
| SHM03 |
100 µl |
MISSION® ExpressMag Beads |
63 |
15 |
1 |
| SHM03 |
300 µl |
MISSION® ExpressMag Beads |
190 |
47 |
4 |
| SHM03 |
1 mL |
MISSION® ExpressMag Beads |
633 |
158 |
15 |
| SHM04 |
1 EA |
MISSION® ExpressMag Super Magnetic Plate |
N/A |
N/A |
N/A |
| SHM05 |
1 EA |
MISSION® ExpressMag 96-Well Magnet |
N/A |
N/A |
N/A |
| Table 1: Average number of transductions achieved with ExpressMag products in commonly used culture dishes. |
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High-throughput Assay Optimization Overview
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Primary Cells Successfully Transduced with ExpressMag Reagents
Figure 2. Primary keratinocytes transduced with MISSION® TurboGFP™ control
lentiviral particles (Product No. SHC003V) using ExpressMag beads with an MOI of 1.
A) Traditional transduction with Polybrene. B) Cells tranduced with ExpressMag beads.
| Primary Cell Type |
Species |
| Airway epithelium | Human, Porcine |
| Aortic Endothelial cells | Human, Bovine |
| Peripheral blood mononuclear cells | Macaques |
| Carotid artery smooth muscle | Bovine |
| Chondrocytes | Porcine |
| Chromaffin | Bovine |
| Cytotrophoblastic | Human, Mouse |
| Embryonic fibroblast | Mouse |
| Epicardial mesothelial | Rat |
| Fibroblast | Human |
| Fibrochondrocyte | Porcine |
| Gastric gland | Human |
| Glial | Human, Mouse, Rat |
| Hippocampal neurons | Mouse, Rat |
| Human umbilical vein endothelial cells | Human |
| Human mammalian epithelial cells | Human |
| Keratinocyte | Human, Mouse |
| Megakaryocyte progenitor | Mouse |
| Nasal airway epithelium | Human |
| Neurons | Mouse, Rat |
| Peripheral blood lymphocytes | Human, Mouse |
| Peripheral blood mononuclear cells | Macaques |
| Rat Hepatocytes | Wistar Rat |
| Smooth muscle | Porcine |
| T lymphocytes (CD4+) | Macaques |
| T lymphocytes (CD8+) | Mouse |
| Trophoblastic | Human |
| Table 2: Primary cells effectively transduced with viral particles using magnetic beads. |
Contact Us
For questions about ExpressMag or other MISSION® lentiviral/RNAi products, please email us at: RNAi@sial.com.
MISSION® ExpressMag™ System FAQ
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
ExpressMag is a trademark of Sigma-Aldrich.
TurboGFP is a trademark of Evrogen.
View the label license of the MISSION® lentiviral shRNA collection.
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