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Sigma-Aldrich is committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on our site, please send us your experimental summary and data by e-mailing MissionRNAi@sial.com.
| Data type |
Format |
Gene Targets |
Cell Lines |
Detection Level |
Provided by |
| Gene Target Validation |
Viral Particles, Transduction |
CDH1 |
Human colorectal cancer cell line (coll5) |
Protein |
Elena Sancho, PhD and Eduard Batlle, PhD
Barcelona Institute for Research in Biomedicine |
| Stable Cell Line/ Signaling Pathway |
Viral Particles, Transduction |
ZFP36 (TTP) |
A549 |
mRNA & Protein |
Kathleen Smoak, PhD and John A Cidlowski, PhD
Laboratory of Signal Transduction, National Institute of Environmental Health Services, National Institutes of Health |
| Efficacy, Specificity and Efficiency |
Viral Particles, Transduction |
p38α, p38γ, JNK2, MAP3K4, JAK1, AKT1, AKT2, AKT3 |
Primary human astrocytes, A549, HepG2, HCT116, Panc1, HeLa |
mRNA |
Sigma-Aldrich R&D |
| Primary Astrocyte Cells (Neuronal Cells) |
Viral Particles, Transduction |
p38α, p38γ, JNK2, MAP3K4 |
Primary human astrocytes |
mRNA |
Sigma-Aldrich R&D |
| Specificity in Targeting Isoforms |
Viral Particles, Transduction |
AKT1, AKT2, AKT3 |
A549 |
mRNA |
Sigma-Aldrich R&D |
| Gene Target Validation |
Viral Particles, Transduction |
c-MYC |
Human lung cancer cell line (H1299) |
mRNA & Protein |
Steven B. McMahon, Ph.D.
The Wistar Institute |
| Demonstration of 3' UTR Utility, Knockdown of Overexpressed Protein |
Plasmid, Transfection |
FBXO6 |
COS-7 |
Protein |
Kevin Glenn, M.D.
Carver College of Medicine, University of Iowa |
| Efficiency of MISSION TurboGFP™ Transduction Control |
Viral Particles, Transduction |
N/A |
Primary BJ Fibroblasts |
Protein (FACS) |
Sheila Stewart, Ph.D.
Washington University |
| Interferon Response |
Viral Particles, Transduction |
CSK, AUR/KB, TYK2, YES1, AKT3 |
HT29 |
mRNA |
Cell, 124, pp. 1283-1298. 20061 |
| Stability of pLKO.1-puro |
Plasmid DNA |
N/A |
N/A |
Restriction Digest |
Cell, 124, pp. 1283-1298. 20061 |
| Dose-Response |
Viral Particles, Transduction |
HCK, PDGFRB, TIE1, BCR, YES1, MAP2K4, PTK7, KIS, PRKACB, MAK |
HT29 |
Cell Viability, Mitotic Index |
Cell, 124, pp. 1283-1298. 20061 |
| Efficacy in Cell Types |
Viral Particles, Transduction |
N/A |
See Table |
N/A |
Compilation |
| High-Throughput Gene Target Validation |
Viral Particles, Transduction |
60 Different Targets |
A549 |
mRNA |
Sigma-Aldrich R&D |
| High-Throughput Screening |
Viral Particles, Transduction |
Human Tumor Suppressor Gene Family Set |
A549 |
Cell Viability Assay |
Sigma-Aldrich R&D |
| High-Throughput Lentiviral Particle Production |
Viral Particles |
2300 individual clones |
N/A |
p24 Assay |
Sigma-Aldrich Operations |
- Moffat J. et al., A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell, 124, pp. 1283-1298. 2006. Request a copy!
Posters
Gene Silencing in Primary Astrocyte Cells using MISSION TRC shRNA Lentiviral Particles
(338 KB PDF)
Efficient Gene Silencing with High Selectivity using MISSION shRNA Lentiviral Vectors
Send this page to a colleague.
Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.
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