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Dose-Response Effects on Cell Number and Mitotic Effect
(A) Viral Dose titration of lentiviruses for control shRNAs. Cell counts determined by automated image analysis following puromycin selection are plotted versus the volume of virus used in the infection.
(B and C) A selection of shRNAs from the primary mitotic index screen in HT29 cells, some of which caused an increase in mitotic index, were used to assess the effect of viral dose on (B) cell counts and (C) mitotic index.
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