Test Interferon Induction by Selected Hits from Primary Screen
The expression levels of several interferon response genes (IFNB1, OAS1, and OAS2) were quantified following transduction of HT29 cells with shRNA-expressing lentivirus. Levels of interferon response gene expression were scaled to an uninfected HT29 control sample (expression=1). Induction of interferon response genes is clearly observed in the positive control sample (primary dendritic cells infected with influenza virus), but is not seen following transduction with shRNA-lentivirus. HT29 cells were transduced in 60 mm plates with 0.25 ml or 1 ml lentivirus containing shRNA expression constructs that showed high MI in the screen, or with a control shRNA lentivirus (shCntrl).
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