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What does it mean if I cannot find my target gene in the database?
The collection is currently being cloned. New target genes will be introduced on a quarterly basis
until the library is completed. It is possible your target gene is not in the current collection,
but may be coming in future releases. Also, it is possible that alternate accession numbers or gene
symbols may be used for your gene of interest. Please check NCBI, for up-to-date information. We
currently do not offer custom cloning services. Please contact us at RNAi@sial.com for more information on target sets currently in
production.
What gene family sets are available?
Currently available in bacterial glycerol stock format
Currently available in lentiviral transduction particle format
Additional sets will be made available over the coming months. If your gene set of interest is not yet available, please inquire for advanced custom preparation at RNAi@sial.com. We can assemble custom gene sets at any time.
Why is there always a clone for the 3' UTR region?
The clones that are against the 3' UTR of the target are included so one could rescue the knockdown
phenotype with the introduction of a cDNA clone corresponding to the target. Transcripts from an
exogenous cDNA clone would not be susceptible to silencing compared to the endogenous copy. Many
publications/review committees require such an experiment to verify the observed phenotype. However,
while most target sets have this added feature, not all target sets have a 3' UTR clone.
Send this page to a colleague.
Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.
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