The MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key structural viral packaging genes and a heterologous viral envelope gene, designed to be co-transfected along with MISSION TRC shRNA clones in order to create high-titer pseudotyped lentiviral particles.
Features:
- VSV-G envelope for transduction of virtually any mammalian cell type
- High-titer lentiviral particles
- In vitro or in vivo transduction applications
- Optimized, easy to use protocol
| Product # |
Product Name |
Reactions
96-well plate |
Reactions
60 mm dish |
Add to Cart |
| SHP001 - 0.25 ml |
MISSION Lentiviral Packaging Mix |
250 |
25 |
|
| SHP001 - 1.70 ml |
MISSION Lentiviral Packaging Mix |
1700 |
170 |
|
Viral Delivery Systems
| Feature |
Adenovirus |
AAV* |
Retrovirus |
Lentivirus |
| Infects non-dividing cells |
X |
X |
|
X |
| Stable integration into host genome |
|
X |
X |
X |
| Can be pseudotyped |
|
X |
X |
X |
| Accepts large inserts |
X |
|
X |
X |
| Lack of interferon response |
|
|
X |
X |
| No issue with transcriptional silencing within genome |
N/A |
|
|
X |
*Adeno-Associated Virus
Viruses are uniquely adapted to infect even the most resilient cell lines. Therefore, viral systems have been genetically engineered over the last few decades to safely deliver transgenes of interest to target cells. While each viral system has features that are ideal in certain applications, the lentiviral system is an example of a specific retroviral system that has undergone multiple generations of engineering and modification. Generating viral particles can be challenging. We highly recommend the viral format of MISSION TRC shRNA clones to new and experienced users. Small and large scale generation of lentiviral particles has been perfected by Sigma.
Fig. 1. Recombinant Lentiviral Packaging Process. For enhanced safety, the structural and replication genes necessary to produce viral particles are separated onto multiple plasmids. All wild-type virulence and accessory genes are deleted. MISSION TRC transfer vectors contain a modified, self-inactiving 3’ long terminal repeat (SIN/LTR) which renders the resulting lentiviral particles replication incompetent. Lentiviral particles are packaged in producer cell lines such as HEK 293T cells. Upon co-transfection of the plasmids, all required sequences are available to produce and package a viral particle containing the transgene of interest. Only the region between the viral LTRs of the transfer vector is packaged within the viral capsid.
Fig. 2. Features of the MISSION Lentiviral Packaging System
| Feature |
Result |
| Multi-plasmid approach |
No single plasmid contains all the genes necessary to produce packaged lentivirus. Resultant particles are replication-incompetent. |
| Deletion in U3 portion of 3' LTR which eliminates the promoter-enhancer region |
Avoids promoter interference issues and further negates the possibility of viral replication. |
| Elimination of the majority of lentiviral genes (Δ vpr, vif, vpu and nef) |
Removes virulence genes which are not necessary for shRNA packaging systems. |
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Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.
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