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Sigma's 10X Capillary Electrophoresis Running Buffer was designed to meet the high quality and high throughput demands of today's sequencing labs. As a primary manufacturer and leading supplier of TBE to the gel-based sequencing community Sigma has the know how and large scale capacity to meet your buffer needs. Scientists at Sigma have taken this experience and joined it with today's advancing technologies to optimize a buffer for use with capillary electrophoresis sequencing instruments.
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Features and Benefits
- Cost efficient - high quality buffer at a great value
- Quality control testing of every lot ensures proper pH, conductivity and performance for Capillary Electrophoresis Sequencing
- Compatible with ABI Prism® 3700, 3100 and 310 DNA sequencers
- Room temperature stability for convenient shipping and storage
- Plastic bottles provide easy handling
- 1, 4 and 20 L as well as custom packaging options available
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| Figure 1. Data quality analysis based on PHRED q>20 scores. Average read length of both single-stranded and double-stranded DNA samples were analyzed on the ABI Prism® 3700 under standard run conditions 3 hours at 1,000V, 50 °C. Samples were prepared using BigDye™ terminators with SigmaSpin™ post-reaction clean up.
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Table 1. 10X CE Buffer Savings
| Number of Instruments |
Runs/Day |
Total Runs/week |
Savings per run using Sigma's 10X CE Buffer |
Annual runs |
Annual Savings (US) |
| 5 |
4 |
100 |
$6.25 |
4,800 |
$30,000 |
| 5 |
8 |
200 |
$6.25 |
9,600 |
$60,000 |
| 10 |
4 |
200 |
$6.25 |
9,600 |
$60,000 |
| 10 |
8 |
400 |
$6.25 |
19,200 |
$120,000 |
| 20 |
4 |
400 |
$6.25 |
19,200 |
$120,000 |
| 20 |
8 |
800 |
$6.25 |
38,400 |
$240,000 |
| 30 |
4 |
600 |
$6.25 |
28,800 |
$180,000 |
| 30 |
8 |
1200 |
$6.25 |
57,600 |
$360,000 |
Calculations: Based on 2002 list price for ABI and Sigma 4 L package size.
Assumptions: Volumes based on 1X buffer per run. Run is defined as 1 x 96-well plate. |
Table 2. 10X CE Buffer Performance
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Run Time |
Average PHRED
q>20 bases |
Average total
read length |
| ABI Prism® 3700 |
3.0 (h) |
520 |
800 |
| MegaBACE® |
2.75 (h) |
495 |
760 |
| Data quality analysis based on PHRED q>20 scores and total readable bases. Data was generated from M13, pGEM and pbluescript templates using BigDye™ Terminators and standard run conditions. |
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Product Number
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Product Description
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Quantity
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B4930
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10X Capillary Electrophoresis Running Buffer
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