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 QuickComb™ -96

Automated Sequencing
 

Sample loading and storage all in one convenient comb.

QuickComb™-96 is a sturdy and reliable porous membrane comb that allows for easy bench-top loading (Fig. 1). The sequencing reactions are spotted on individual teeth, the comb is inserted and current applied. Samples are drawn from the porous teeth resulting in maximum signal strength and easier, more consistent lane tracking (Fig. 2).

With QuickComb™-96 you can load your samples and store them directly on the comb for up to 2 weeks (Fig. 3). For long-term storage, samples are stable on QuickComb™-96 for up to 6 months at -20 °C.

 
Features and Benefits
  • Bench-top loading eliminates the need for expensive syringe loaders and reduces user fatigue
  • Increased data quality when compared to standard loading protocols
  • Superior lane tracking capabilities
  • No well to well leaching or loss in signal strength
  • Spot and use your comb immediately or store spotted samples up to 6 months with no compromise in data quality
  • Compatible with ABI Prism® 377 standard or beveled "step-glass" plates
  • Laminated to avoid tearing and bending while inserting the comb

 

Figure 1. Use a single or multi-channel pipette to spot sequencing reactions on individual teeth. Insert the comb into the gel and electrophorese.


Figure 2. QuickComb™-96 eliminates lane-to-lane leaching and allows for easier lane tracking.

Panel A. Samples loaded on adjacent teeth of QuickComb™-96. Both single- and double- stranded DNA templates were loaded. All samples were run on AutoPAGE™ Plus 4.5% gels using standard ABI 36E-1200 Run Module. Panel B. Samples loaded directly onto cells in alternating lanes to combat lane-to-lane leakage. Both single- and double-stranded DNA templates were run on AutoPAGE™ Plus 4.5% gels using standard ABI 36E-1200 Run Module.

Figure 3. Analysis for samples loaded and stored for 2 weeks versus freshly loaded samples based on PHRED q>20 scores. Control samples were single-stranded DNA sequenced with ABI BigDye™ Terminators. Samples were run on AutoPAGE™ Plus 4.5% gels using BigDye™ standard ABI 36E-1200 Run Module.

Ordering Information
Product Number Product Description Quantity
C3226 QuickComb™-96 Porous membrane loading and storage comb
10 each
50 each
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