SigmaSpin™ Post-Reaction Clean-Up

SigmaSpin™ Post-Reaction Clean-Up Columns

SigmaSpin™ Post-Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean-up of probe labeling reactions or small numbers of sequencing reactions.

These columns can accept sample volumes up to
100 µl. Each column comes with a collection tube to collect the DNA during centrifugation.

SigmaSpin™ 96-Well Post-Reaction Clean-Up plates provide a fast, simple, and highly efficient method for removing unincorporated dyes, excess salts, and other interfering reaction components (Fig. 1). Each plate is packed with a pre-hydrated size-exclusion resin, equilibrated with molecular biology grade water, and supplied in our unique plate design with long drip directors to minimize contamination between samples. The plate design also includes a snap-cap bottom seal and a foil seal top to ensure that the resin remains hydrated. SigmaSpin™ has been tested in high-throughput genome centers and core facilities with ABI Prism® 3700, 3100, 310 and 377. Each well can accept sample volumes up to 20 µl.

• Dye-terminator nucleotides and primers from sequencing reactions
• Radiolabeled nucleotides, primers, and fluorescent dyes from nucleic acid probe labeling reactions

• Validated with most automated sequencers and dye terminator chemistries (Fig. 2)
• Pre-qualified size-exclusion resin guarantees optimum performance
• Unique drip directors prevent cross-contamination between samples during collection
• Plates are sealed to eliminate leakage or drying during shipping or storage
• Suitable for use with multi-channel pipettes and automated workstations