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Cloning & Expression
 
 
Equipment & Supplies
Lysing Enzymes
Media Components
Modifying Enzymes
Prepared Media
Restriction Endonucleases
Restriction Endonuclease Buffers

Equipment & Supplies  
Product Name Product # 
Culture flasks with vented cap, Size: 75 cm2 Z35,344-2
Culture flasks with vented cap, Size: 150 cm2 Z35,345-0
Culture Flask Fernbach, Size: 2.8 L, polycarbonate, Autoclavable; repeated autoclaving may reduce mechanical strength Z36,023-6
Petri-Pad Culture Dish System, complete unit, Unit consists of a 47 mm culture dish and one Millipore pure cellulose Petri-Pad C6695
Petri-Pad Culture Dish System, culture dishes only C6570
Corning Culture Dishes, Size: 100 mm × 20 mm, Non-treated C6796
Culture Tubes, Size: 12 mm × 75 mm T1661
Petri Dishes, Polystyrene, Size: 60 mm × 15 mm, Beveled stacking rings P5481
Petri Dishes, Polystyrene, Size: 100 mm × 20 mm, Beveled stacking rings P5606
Petri Dishes, Polystyrene, Size: 100 mm × 15 mm, Vertical stacking rings P5856
Petri Dishes, Polystyrene, Size: 150 mm × 15 mm, Ringless for easy sliding P5981
Inoculating Loops, Loop : 3 mm, Nichrome wire, aluminum handle I8763
Lazy-L Spreaders, polystyrene, Sterile Z37,677-9
Inoculating Loop/Needle, Loop : 1 µL I8263
Stripette Serological Pipettes , 1 mL Z37,158-0
Stripette Serological Pipettes , 2 mL Z37,159-9
Stripette Serological Pipettes , 5 mL Z37,160-2
Stripette Serological Pipettes , 10 mL Z37,161-0
Stripette Serological Pipettes , 25 mL Z37,162-9
SigmaWare Pasteur Pipettes, Cotton plugged, Size: 5-3/4 in. S5893
SigmaWare Pasteur Pipettes, Cotton plugged, Size: 9 in. S6143
SigmaWare Pasteur Pipettes, Non-plugged, Size: 5-3/4 in. S6018
SigmaWare Pasteur Pipettes, Non-plugged, Size: 9 in. S6268
Sterilizer, infrared heat, 120V AC S3398
Sterilizer, infrared heat, 240V AC S3273
Replica Plater for 96 well plate, 8 x 6 Array R2383
Replica Plater for 96 well plate, 8 x 12 Array R2508
Replicator, 96-well, Autoclavable Z37,081-9
Replica Plater for nutrient plate Z36,339-1
Velveteen Squares for Replica Plater Z36,340-5
Replica Plating Grid, polystyrene, Accepts 100 mm Petri plates Z36,338-3
Easi-Grid Master Plating Device, red, with grid set Z37,046-0
Easi-Grid Master Plating Device, Yellow tint, with grid set Z37,047-9
Easi-Grid Master Plating Device, blue, with grid set Z37,045-2
Easi-Grid Master Plating Device, black, with grid set Z37,044-4
Stand for Easi-Grid Plating Device, Capacity: 4 plating devices Z37,049-5
Nalgene® Cryogenic Vials, Size: 2.0 mL, Sterile V5007
Nalgene® Cryogenic Vials, Size: 1.2 mL, Sterile V4757
Nalgene® Cryogenic Vials, System 100, Size: 1.5 mL Z35,903-3
Rack for Nalgene Cryogenic Vials, white, Holds 50 vials in a 5 × 10 array. R0638
Rack for Nalgene Cryogenic Vials, blue, holds 25 vials in a staggered array. Z35,904-1
Nalgene® CryoBox, 5 x 5 Array For 1.2 and 2.0 ml vials R0763
Nalgene® CryoBox, 9 x 9 Array For 1.2 and 2.0 ml vials R0888
Shake Flasks, triple baffled, Size: 250 mL F0644
Shake Flasks, triple baffled, Size: 1000 mL F0769
Silicone Sponge Closures, Size: 28 mm C0921
Silicone Sponge Closures, Size: 38 mm C1046
Test Tube Racks, Capacity: 24 25-mm tubes 3 × 8 array R3633
Rack, centrifuge tube, epoxy-coated steel, for 50 ml plastic centrifuge tubes, 8-Position R8509
Rack, Microcentrifuge Tube, Two-tiered, 10 positions per tier, Autoclavable, polypropylene R6630
Rack, Microcentrifuge Tube, Insert holds: 72 0.5-ml tubes R4636
Rack, Microcentrifuge Tube, Insert holds: 50 1.5-ml tubes R4511
Lysing Enzymes back to top
Product Name Product # 
Lysozyme from chicken egg white, Lyophilized powder, approx. 50,000 units/mg protein (E2801%) L7651
Lyticase Escherichia coli, recombinant, expressed in Escherichia coli, Lyophilized powder L4276
Lyticase from Arthrobacter luteus, Lyophilized powder, 5,000-20,000 units/mg protein L2524
Lysing Enzymes from Trichoderma harzianum, Lyophilized powder L1412
Mutanolysin from Streptomyces globisporus ATCC 21553, 4,000-8,000 units/mg protein (modified Warburg-Christian) M9901
Media Components back to top
Product Name Product # 
Protein Hydrolysates  
Amicase, bovine milk A2427
Hy-Case Amino, bovine milk C0501
Bacteriological Peptone P0556
Lactalbumin Enzymatic Hydrolysate L0375
Hy-Case SF, bovine milk C9386
EZMixä Hy-Case SF C4589
N-Z-Amine A, bovine milk C0626
EZMixä N-Z-Amine A C4464
N-Z-Amine AS, bovine milk N4517
N-Z-Case Plus, bovine milk N4642
N-Z-Case TT, bovine milk C4523
N-Z-Soy Peptone P1265
Primatone P8388
Proteose Peptone P0431
Tryptone T9410
EZMixä Tryptone T2559
Agars back to top
Select agar A5054
Bacteriological agar A5306
Noble agar A5431
Extracts back to top
Beef extract powder B4888
Malt Extract M0383
Yeast extract, alternative to beef extract, Powder Y1625
Yeast Extract EZMixä Powder, alternative to beef extract Y1626
Media Reagents back to top
Antifoam A Emulsion A6457
Antifoam B Emulsion A6707
D-(+)-Glucose anhydrous, component for culture media, minimum 99% (GC) G5400
Glycerol anhydrous, component for culture media, minimum 99% (GC) G5150
b-Lactose, component for culture media L8773
3-Indoleacrylic acid, minimum 99% (TLC) I2273
Isopropyl b-D-1-thiogalactopyranoside, minimum 99% (TLC) I6758
Isopropyl b-D-1-thiogalactopyranoside, minimum 99% (TLC) I5502
Reporter Gene Substrates back to top
Blue-White Selectä Screening Reagent B3928
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, identification of lac+ bacterial colonies, minimum 98% B9146
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, identification of lac+ bacterial colonies, Tablet B6024
5-Bromo-4-chloro-3-indolyl b-D-glucuronide sodium salt, minimum 98% (TLC) B4782
5-Bromo-4-chloro-3-indolyl b-D-glucuronide sodium salt, Tablet B8174
5-Bromo-4-chloro-3-indolyl b-D-glucuronide cyclohexylammonium salt, minimum 98% (TLC) B6650
5-Bromo-4-chloro-3-indolyl b-D-glucuronide cyclohexylammonium salt, Tablet B8049
5-Bromo-3-indolyl b-D-galactopyranoside B2904
5-Bromo-6-chloro-3-indolyl b-D-glucuronide cyclohexylammonium salt, minimum 98% B4657
4-Methylumbelliferyl-b-D-galactopyranoside, minimum 99% (TLC) M1633
4-Methylumbelliferyl b-D-glucuronide hydrate, minimum 98% (TLC) M9130
2-Nitrophenyl b-D-galactopyranoside, minimum 98% (enzymatic) N1127
4-Nitrophenyl b-D-glucuronide, minimum 98% (TLC) N1627
2-Nitrophenyl 1-thio-b-D-galactopyranoside N2509
Phenyl-b-D-galactopyranoside, minimum 98% (TLC) P6501
Modifying Enzymes back to top
Product Name Product # 
Agarase from Pseudomonas atlantica, suitable Tested for suitability in isolating l DNA Hind III fragments from low-melting agarose gels (Functional assay), Buffered aqueous glycerol solution A8688
DNA Polymerase I solution from Escherichia coli lysogenic for NM 964, 5,000-15,000 units/mL, Buffered aqueous glycerol solution D9380
DNA Polymerase I, Klenow Fragment from Escherichia coli, approx. 5,000 units/mL, Buffered aqueous glycerol solution D8276
DNA Polymerase T4 from Escherichia coli 71-18/pTL43W, 5,000-20,000 units/mL, Buffered aqueous glycerol solution D0410
REDTaqä DNA Polymerase, 1 unit/µL D4309
REDTaqä Genomic DNA Polymerase without MgCl2, 1 unit/µL D2812
Taq DNA Polymerase from Thermus aquaticus, 5 units/µL, PCR amplification and cycle sequencing, recombinant, expressed in Escherichia coli D1806
Taq DNA Polymerase from Thermus aquaticus, 5 units/µL, PCR amplification and cycle sequencing, recombinant, expressed in Escherichia coli, Solution without MgCl2 D4545
Uracil DNA Glycosylase from Escherichia coli U1257
Polynucleotide Kinase from T4-infected Escherichia coli, approx. 5,000 units/mL, Buffered aqueous glycerol solution P4390
DNA Ligase from T4-infected Escherichia coli, 500-6,000 units/mL, Buffered aqueous glycerol solution D2886
Deoxyribonuclease I RNase-free solution from bovine pancreas, Buffered aqueous glycerol solution, ³10,000 units/mg protein D7291
Ribonuclease A from bovine pancreas, Lyophilized powder, ³70 units/mg protein R6513
Ribonuclease A from bovine pancreas, Buffered aqueous glycerol solution, ³70 units/mg protein R4642
Phosphatase, Alkaline preparation from Escherichia coli, Buffered aqueous glycerol solution, 20-50 units/mg protein, pH 10.4, 37°C (glycine buffer) P4069
Phosphatase, Alkaline preparation from Escherichia coli, 30-90 unit/mg protein (modified Warburg-Christian, in glycine buffer) P4252
Protease Streptomyces griseus, approx. 4 units/mg solid P6911
Proteinase K Tritirachium album, Lyophilized powder, >30 units/mg protein P2308
Proteinase K Tritirachium album, >800 units/mL, Buffered aqueous glycerol solution P4850
Proteinase K-Agarose from Tritirachium album, Lyophilized powder P9290
rec A Protein from Escherichia coli strain GE 1171/pGE226, 1-5 mg/ml in 20 mM Tris acetate, pH 7.5, 50% glycerol, 0.1 mM EDTA and 1 mM DTT, minimum 0.25 unit/mg protein R7272
AMV-Reverse Transcriptase avian myeloblastosis virus, 10,000-100,000 units/mL R9376
Enhanced Avian Reverse Transcriptase from avian myeloblastosis virus A4464
M-MLV Reverse Transcriptase M1302
RNA Polymerase SP6 solution from SP6-infected Salmonella typhimurium, 10,000-50,000 units/mL, Buffered aqueous solution R0759
RNA Polymerase T7 Recombinant solution from Escherichia coli, 10,000-50,000 units/mL, recombinant, expressed in Escherichia coli, Buffered aqueous solution R0884
Poly(A) Polymerase from Escherichia coli, 1,000-5,000 units/mL, Buffered aqueous glycerol solution P4058
Topoisomerase I from wheat germ, Buffered aqueous glycerol solution T7428
Terminal Transferase from calf thymus, ³5,000 units/mL, Buffered aqueous glycerol solution T4427
Prepared Media back to top
Product Name Product # 
Escherichia Coli Media  
LB Agar, Powder L2897
LB Agar EZMixä Powder L7533
LB Agar, Tablet L7025
S-Galä/LB Agar Blend C4478
LB Broth, Liquid L2542
LB Broth, Powder L3022
LB Broth EZMixä Powder L7658
LB Broth, Tablet L7275
Luria Agar L3147
Luria Agar Base (Miller's Modification) L3272
Luria Broth L3522
Luria Broth (Miller's Modification) L3397
NZ Broth N3518
NZCYM Broth, Powder N3643
NZCYM Broth EZMixä Powder N6905
M9 Minimal Salts, 5X M6030
Hanahan's Broth H8032
SOC Medium S1797
Terrific Broth, Liquid T5574
Terrific Broth, modified, Powder T0918
Terrific Broth, Modified EZMixä Powder T9179
2X YT Microbial Medium, Powder Y2377
2X YT Microbial Medium EZMixä Powder Y2627
Yeast Media back to top
YPD Agar Y1500
YPD Broth Y1375
Yeast Nitrogen Base With Amino Acids Y1250
Yeast Nitrogen Base without amino acids and ammonium sulfate Y1251
Yeast Nitrogen Base without amino acids Y0626
Yeast Synthetic Drop-out Medium Supplement Without Histidine Y1751
Yeast Synthetic Drop-out Medium Supplement Without Leucine Y1376
Yeast Synthetic Drop-out Medium Supplement Without Tryptophan Y1876
Yeast Synthetic Drop-out Medium Supplement Without Leucine and Tryptophan Y0750
Yeast Synthetic Drop-out Medium Supplement Without Uracil Y1501
Yeast Synthetic Drop-out Medium Supplement Without Histidine, Leucine, Tryptophan and Uracil Y2001
Restriction Endonucleases back to top
Product Name Product # 
Restriction Endonuclease Acc I from Acinetobacter calcoaceticus, minimum 5,000 units/mL, Buffered aqueous glycerol solution R6142
Restriction Endonuclease Afl III from Anabaena flos-aquae, 5,000 units/mL, Buffered aqueous glycerol solution R1259
Restriction Endonuclease Alu I from Arthrobacter luteus, 10,000 units/mL, Buffered aqueous glycerol solution R6885
Restriction Endonuclease Alw44 I from Acinetobacter lwoffii, 10,000 units/mL, Buffered aqueous glycerol solution R6634
Restriction Endonuclease Apa I from Acetobacter pasteurianus, 10,000 units/mL, Buffered aqueous glycerol solution R4258
Restriction Endonuclease Ava I from Anabaena variabilis, 5,000 units/mL, Buffered aqueous glycerol solution R3379
Restriction Endonuclease Ava II from Anabaena variabilis, 5,000 units/mL, Buffered aqueous glycerol solution R6004
Restriction Endonuclease BamH  I from Bacillus amyloliquefaciens H, 10,000 units/mL, Buffered aqueous glycerol solution R0260
Restriction Endonuclease Ban II from Bacillus aneurinolyticus (Bacillus brevis), 10,000 units/mL, Buffered aqueous glycerol solution R2630
Restriction Endonuclease Bcl I from Bacillus caldolyticus, 10,000 units/mL, Buffered aqueous glycerol solution R8631
Restriction Endonuclease Bgl I from Bacillus globigii (Bacillus licheniformis), 10,000 units/mL, Buffered aqueous glycerol solution R6753
Restriction Endonuclease Bgl II from Bacillus globigii (Bacillus licheniformis), 10,000 units/mL, Buffered aqueous glycerol solution R6377
Restriction Endonuclease Bln I from Brevibacterium linens, 10,000 units/mL, Buffered aqueous glycerol solution R3131
Restriction Endonuclease Bsm I from Bacillus stearothermophilus NUB 36, 10,000 units/mL, Buffered aqueous glycerol solution R3635
Restriction Endonuclease BssH II from Bacillus stearothermophilus, 10,000 units/mL, Buffered aqueous glycerol solution R2634
Restriction Endonuclease BstE II from Bacillus stearothermophilus ET, 10,000 units/mL, Buffered aqueous glycerol solution R4253
Restriction Endonuclease Cfo I from Clostridium formicoaceticum, 10,000 units/mL, Buffered aqueous glycerol solution R1761
Restriction Endonuclease Cla I from Caryophanon latum L, 10,000 units/mL, Buffered aqueous glycerol solution R7763
Restriction Endonuclease Dde I from Desulfovibrio desulfuricans strain Norway, 10,000 units/mL, Buffered aqueous glycerol solution R4256
Restriction Endonuclease Dpn I from Diplococcus pneumoniae, 10,000 units/mL, Buffered aqueous glycerol solution R8381
Restriction Endonuclease Dra I from Deinococcus radiophilus, 10,000 units/mL, Buffered aqueous glycerol solution R4381
Restriction Endonuclease EclX I from Enterobacter cloacae 590, 10,000 units/mL, Buffered aqueous glycerol solution R3884
Restriction Endonuclease EcoR I from Escherichia coli BS5, 10,000 units/mL, Buffered aqueous glycerol solution R6265
Restriction Endonuclease EcoR I from Escherichia coli BS5, 40,000 units/mL, Buffered aqueous glycerol solution R4640
Restriction Endonuclease EcoR II from Escherichia coli MV1 193, 10,000 units/mL, Buffered aqueous glycerol solution R1636
Restriction Endonuclease EcoR V from Escherichia coli, 10,000 units/mL, Buffered aqueous glycerol solution R2756
Restriction Endonuclease Hae II from Haemophilus aegyptius, 3,000-10,000 units/mL, Buffered aqueous glycerol solution R4257
Restriction Endonuclease Hae III from Haemophilus aegyptius, 10,000 units/mL, Buffered aqueous glycerol solution R5628
Restriction Endonuclease Hind II from Haemophilus influenzae Rd, 3,000-10,000 units/mL, Buffered aqueous glycerol solution R1382
Restriction Endonuclease Hind  III from Haemophilus influenzae, 10,000 units/mL, Buffered aqueous glycerol solution R1137
Restriction Endonuclease Hpa I from Haemophilus parainfluenzae, 3,000-10,000 units/mL, Buffered aqueous glycerol solution R8507
Restriction Endonuclease Hpa II from Haemophilus parainfluenzae, 10,000 units/mL, Buffered aqueous glycerol solution R0629
Restriction Endonuclease Kpn I from Klebsiella pneumoniae, 10,000 units/mL, Buffered aqueous glycerol solution R1258
Restriction Endonuclease Ksp I from Kluyvera sp., 10,000 units/mL, Buffered aqueous glycerol solution R4134
Restriction Endonuclease Mlu I from Micrococcus luteus (lysodeikticus), 10,000 units/mL, Buffered aqueous glycerol solution R8257
Restriction Endonuclease MluN I from Micrococcus luteus strain N, 10,000 units/mL, Buffered aqueous glycerol solution R1507
Restriction Endonuclease Msp I from Moraxella sp., 10,000 units/mL, Buffered aqueous glycerol solution R4506
Restriction Endonuclease Mva I from Micrococcus varians Rfl 19, 8,000-12,000 units/mL, Buffered aqueous glycerol solution R1632
Restriction Endonuclease Nci I from Neisseria cinerea, 10,000 units/mL, Buffered aqueous glycerol solution R5635
Restriction Endonuclease Nco I from Nocardia corallina, 10,000 units/mL, Buffered aqueous glycerol solution R8761
Restriction Endonuclease Nde I from Neisseria denitrificans, 10,000 units/mL, Buffered aqueous glycerol solution R5509
Restriction Endonuclease Nde II from Neisseria denitrificans NRCC 31009, 5,000 units/mL, Buffered aqueous glycerol solution R5757
Restriction Endonuclease Nhe I from Neisseria mucosa heidelbergensis, 10,000 units/mL, Buffered aqueous glycerol solution R5634
Restriction Endonuclease Not I from Nocardia otidiscaviarum, 10,000 units/mL, Buffered aqueous glycerol solution R8506
Restriction Endonuclease Nru I from Nocardia rubra, 10,000 units/mL, Buffered aqueous glycerol solution R5759
Restriction Endonuclease Nsi I from Neisseria sicca, 10,000 units/mL, Buffered aqueous glycerol solution R5884
Restriction Endonuclease Pst I from Providencia stuartii, 10,000 units/mL, Buffered aqueous glycerol solution R7023
Restriction Endonuclease Pvu I from Proteus vulgaris, 5,000 units/mL, Buffered aqueous glycerol solution R1508
Restriction Endonuclease Pvu II from Proteus vulgaris, 10,000 units/mL, Buffered aqueous glycerol solution R2631
Restriction Endonuclease Rsa I from Rhodopseudomonas sphaeroides, 10,000 units/mL, Buffered aqueous glycerol solution R4756
Restriction Endonuclease Sac I from Streptomyces achromogenes, 10,000 units/mL, Buffered aqueous glycerol solution R5268
Restriction Endonuclease Sal I from Streptomyces albus G, 10,000 units/mL, Buffered aqueous glycerol solution R0754
Restriction Endonuclease Sau3A I from Staphylococcus aureus, 10,000 units/mL, Buffered aqueous glycerol solution R0762
Restriction Endonuclease Sca I from Streptomyces caespitosus, 10,000 units/mL, Buffered aqueous glycerol solution R5007
Restriction Endonuclease ScrF I from Streptococcus cremoris, 10,000 units/mL, Buffered aqueous glycerol solution R7888
Restriction Endonuclease Sfi I from Streptomyces fimbriatus, 10,000 units/mL, Buffered aqueous glycerol solution R8256
Restriction Endonuclease Sma I from Serratia marcescens Sb, 10,000 units/mL, Buffered aqueous glycerol solution R4503
Restriction Endonuclease Spe I from Sphaerotilus sp., 10,000 units/mL, Buffered aqueous glycerol solution R5257
Restriction Endonuclease Sph I from Streptomyces phaeochromogenes, 10,000 units/mL, Buffered aqueous glycerol solution R7135
Restriction Endonuclease Ssp I from Sphaerotilus sp., 10,000 units/mL, Buffered aqueous glycerol solution R6759
Restriction Endonuclease Stu I from Streptomyces tubercidicus, 10,000 units/mL, Buffered aqueous glycerol solution R8013
Restriction Endonuclease Sty I from Escherichia coli strain carrying pST27, 10,000 units/mL, Buffered aqueous glycerol solution R7009
Restriction Endonuclease Swa I from Staphylococcus warneri, 10,000 units/mL, Buffered aqueous glycerol solution R1885
Restriction Endonuclease Taq I, 10,000 units/mL, Buffered aqueous glycerol solution R9507
Restriction Endonuclease Xba I from Xanthomonas badrii, 10,000 units/mL, Buffered aqueous glycerol solution R7260
Restriction Endonuclease Xho I from Xanthomonas holcicola, 10,000 units/mL, Buffered aqueous glycerol solution R6379
Restriction Endonuclease Buffers back to top
Product Name Product # 
Restriction Endonuclease Buffer SA, 10 x B7531
Restriction Endonuclease Buffer SB, 10 x B8781
Restriction Endonuclease Buffer SL, 10 x B3782
Restriction Endonuclease Buffer SM, 10 x B3158
Restriction Endonuclease Buffer SH, 10 x B3657
Buffer SP, 2 x B6048

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