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High Efficiency Competent Cells for your Sensitive Cloning Applications!
Sigma-Aldrich offers a wide range of chemically competent cells as well as Thunderbolt™ GC10™ electrocompetent cells. Our competent cells are offered at the highest transformation efficiencies for sensitive cloning applications. In addition, we offer a variety of strains to meet your specific needs for protein expression and plasmid propagation.
Features and Benefits
- Convenient Unipack formats for one time use
- Strains comparable to DH5α™ and DH10B™
- High efficiencies
- T1 phage resistance
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Other Resources:
Watch the new technical presentation "Everything you Need to Know about Competent Cells!"
Calculating Transformation Efficiences
Genotypes, Phenotypes and Markers - Definitions
E. coli Markers
Strains of E. coli
Cloning Strains
Features and Benefits
- F' episome for single strand rescue
- Strains for cloning methylated DNA
- Blue/white selection
| Product # |
Product Name |
Size |
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G3169 |
GC5™ Competent Cells, Uni-pack |
10 x 50 µl
20 x 50 µl |
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G3044 |
GC5™ Competent Cells, Standard Aliquots |
5 x 200 µl |
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G7419 |
GC5™ Competent Cells, 96-well |
1 EA
4 EA |
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G2919 |
GC10™ Competent Cells, Uni-Pack |
10 x 50 µl
20 x 50 µl |
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G2794 |
GC10™ Competent Cells, Standard Aliquots |
5 x 200 µl |
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T7699 |
Thunderbolt™ GC10™ ElectroCompetent Cells |
5 x 80 µl
5 x 100 µl |
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J3895 |
JM109 Competent Cells, Uni-pack |
10 x 50 µl |
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H3788 |
HB101 Competent Cells, Uni-pack |
10 x 50 µl |
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Expression Strains
Features and Benefits
- T1 phage resistance
- Strains for T7 systems
- Ion and omp T protease deficient
| Product # |
Product Name |
Size |
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B2685 |
BL21-T1R Competent Cells, Uni-pack |
10 x 50 µl |
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B2935 |
BL21 (DE3)-T1R Competent Cells, Uni-pack |
10 x 50 µl |
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B3310 |
BL21 (DE3) pLysS-T1R Competent Cells, Uni-pack |
10 x 50 µl |
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B3435 |
BL21 (DE3) pLysE-T1R Competent Cells, Uni-pack |
10 x 50 µl |
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Competent Cell Strain Selection Table:
| Feature |
GC5™ |
GC10™ |
Thunderbolt™ GC10™ |
JM109 |
HB101 |
BL21 |
BL21 (DE3) |
BL21 (DE3) pLysS |
BL21 (DE3) pLysE |
| Comparable to DH5a™ |
X |
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| Comparable to NovaBlue and XL1 Blue |
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X |
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| Comparable to DH10B™ |
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X |
X |
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| Strain Background |
K12 |
K12 |
K12 |
K12 |
K12xB |
B |
B |
B |
B |
| Transformation Efficiency (cfu/ug pUC plasmid DNA) |
109 |
109 |
1010 |
108 |
108 |
106 |
107 |
106 |
106 |
| Cloning Strain |
X |
X |
X |
X |
X |
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| Expression Strain |
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X |
X |
X |
X |
| Blue/White Selection (with appropriate construct) |
X |
X |
X |
X |
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| T1 bacteriophage resistant |
X |
X |
X |
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X |
X |
X |
X |
| T7 promoter systems |
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X |
X |
X |
| F' episome for single strand rescue |
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X |
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| Ideal for cloning methylated DNA |
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X |
X |
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X |
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| lacI q (higher level of lac repressor) |
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X |
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GC5™, GC10™, and Thunderbolt™ are trademarks of GeneChoice ®, Inc.
DH5α™ and DH10B™ are trademarks of Invitrogen Corp.
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