Order Center
 
Life Science

Antibodies
Automation
Cancer Research
Cell Culture
Cell Signaling and Neuroscience
Custom Synthesis
Drug Discovery
Epigenetics
Functional Genomics and RNAi
Metabolomics
Molecular Biology
Automated Sequencing
Cloning and Expression
Key Resources
DNA and RNA Purification
Gene Expression Analysis
Molecular Biology Reagents
Nucleic Acid Detection
Nucleic Acid Electrophoresis
PCR
Whole Genome Amplification
New Products
Neuroscience
Nutrition Research
Obesity Research
Peptides and Proteins
Plant Biotechnology
Proteomics and Protein Expr.
Stable Isotopes
Stem Cell Biology
Your Favorite Gene - Search
Life Science Innovations
PathFinder

 Expression Vectors

Cloning & Expression
 

DNA vectors are the foundation of recombinant protein expression. Sigma now offers such vectors for bacterial and mammalian expression. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.

  • Our exclusive FLAG® and 3xFLAG® tags are provided in N-terminal, C-terminal, and dual tag configurations for detection and purification using ANTI-FLAG® antibodies, resins, and 96-well plates.
  • Unique BICEP™ vectors contain an optimized EMCV IRES for high levels of bicistronic expression for optimal maintenance of stable cell lines and multi-gene expression.
  • The new MAT™ (Metal Affinity Tag) has been incorporated in many of our popular vectors for high quality purification using HIS-Select™ affinity gels.

Whatever your protein and expression needs, Sigma has the vector for you. Click on the links for information on each vector.


Bacterial Expression Vectors

Product # 
Product Name
Size
E5769
pFLAG-ATS™
10µg
E5644
pFLAG-MAC™
10µg
E5269
pFLAG-CTS™
10µg
E5394
pFLAG-CTC™
10µg
E8023
pFLAG-Shift12
10µg
E7898
pFLAG-Shift12c
10µg
E5530
pTAC-MAT™-1
10µg
E5405
pTAC-MAT™-2
10µg
P1118
pT7-FLAG™-1
10µg
P1243
pT7-FLAG™-2
10µg
E5780
pT7-MAT™-1
10µg
E5655
pT7-MAT™-2
10µg
E5280
pT7-FLAG-MAT™-1
10µg
E4905
pT7-MAT-FLAG™-2
10µg
E5155
pT7-MAT-FLAG™-1
10µg
E5030
pT7-FLAG-MAT™-2
10µg
P9618
pT7-FLAG™-3
10µg
P9743
pT7-FLAG™-4
10µg

back to top


Mammalian and BICEP™ Expression Vectors

Transient Expression

Product # 
Product Name
Size
E7273
pFLAG-CMV™-1
20µg
E7398
pFLAG-CMV™-2
20µg
E7901
pFLAG-CMV™-5.1
20µg
E3762
pFLAG-CMV™-5a,b,c
1 set
E2275
pFLAG-CMV™-6a,b,c
1 set
E4026
p3xFLAG-CMV™-7.1
20µg
E4151
p3xFLAG-CMV™-8
20µg
E5526
pFLAG-myc-CMV™-19
20µg
E5651
pFLAG-myc-CMV™-20
20µg
E6026
p3xFLAG-myc-CMV™-23
20µg
E6151
p3xFLAG-myc-CMV™-24
20µg
C5864
pCMV-FLAG-MAT™-1
20µg
C5989
pCMV-MAT-FLAG™-1
20µg
C6114
pCMV-FLAG-MAT™-2
20µg
E7029
pBICEP-CMV™-3
20µg
E5905
pCMV-BICEP™-4
20µg

Stable Expression

Product # 
Product Name
Size
E8770
pFLAG-CMV™-3
20µg
E1775
pFLAG-CMV™-4
20µg
E4276
p3xFLAG-CMV™-9
20µg
E4401
p3xFLAG-CMV™-10
20µg
E4776
p3xFLAG-CMV™-13
20µg
E4901
p3xFLAG-CMV™-14
20µg
E5776
pFLAG-myc-CMV™-21
20µg
E5901
pFLAG-myc-CMV™-22
20µg
E6276
p3xFLAG-myc-CMV™-25
20µg
E6401
p3xFLAG-myc-CMV™-26
20µg
E0779
pBICEP-CMV™-1
20µg
E0904
pBICEP-CMV™-2
20µg

back to top
back to Cloning & Expression


Site Use Terms Terms and Conditions of Sale Privacy Business Development Contact Us
Copyrights © 2008 Sigma-Aldrich Co. All Rights Reserved.
Reproduction of any materials from the site is strictly forbidden without permission.
Sigma-Aldrich brand products are sold exclusively through Sigma-Aldrich, Inc.