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S-Gal™/LB Agar Blends for Detecting Gene Insertions |
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Cloning & Expression |
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S-Gal™ a new chromogen to detect gene insertions: |
- Contains a patented chromogenic substrate,
S-Gal (3,4-cyclohexenoesculetin-b-D-galacto-
pyranoside).
- Replaces X-gal as the preferred substrate for
measurement of b-galactosidase transformed
cells used in molecular biology applications.
- Hydrolyzed by b-galactosidase to yield
an
intense black insoluble precipitate.
- Heat and light stable, non-toxic.
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S-Gal, a new chromogenic substrate
for gene insertion detection.
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S-Gal/LB Agar Blend
Product. No. C 4478 is a complete powder mixture useful for detecting prokaryotic cells which
have been transformed with vectors containing the lacZ gene. It contains S-Gal, a new chromogenic
substrate, and isopropyl b-D-thio-galactopyranoside, (IPTG).
IPTG initiates the transcription of the lacZ gene
by binding to the lac repressor
product of the
Lac I gene and allowing the lac operator to turn on transcription.
The product
of the lacZ gene is b-galactosidase.
b-Galactosidase transformed
cells have traditionally been measured by the
hydrolysis of X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactoside) to a blue precipitate.
Now we have S-Gal!
| FEATURES |
BENEFITS |
| Faster colony color development. |
Pick colonies for faster results. |
| Lac+
colonies turn an intense black color. |
Easier to distinguish between lac + and lac - colonies.
May improve accuracy of automated colony pickers. |
| Complete with LB Agar, S-Gal, IPTG |
No need to make up separate solutions of X-gal and IPTG, saving time and effort.
No need to wait for media to cool before adding X-gal and IPTG.
No need for toxic solvents, such as DMF. |
| Convenient packaging. Each pouch holds the required amount of S-Gal/LB Agar Blend powder to make up to 500 ml of media. |
Ease of use for the researcher. Just add pre-blended powder to 500 ml of water, autoclave, add antibiotic if desired, and pour plates.
Saves time and labor. |
| S-Gal is not heat labile. |
Can be autoclaved or microwaved.
Provides flexibility to user. |
| S-Gal is not light sensitive. |
No need to shield product or plates from light.
Consistent performance and reliable results. |
Just add water to S-Gal/LB Agar Blend, autoclave and pour your plates. It's that easy!
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S-Gal/Kanamycin/LB Agar Blend
Product. No. S 1813 is a complete powder mixture useful for detecting prokaryotic cells which
have been transformed with vectors containing the lacZ gene. It contains S-Gal, kanamycin,
and isopropyl b-D-thio-galactopyranoside, (IPTG).
| FEATURES |
BENEFITS |
| Faster colony color development. |
Pick colonies for faster results. |
| Lac+
colonies turn an intense black color. |
Easier to distinguish between lac + and lac - colonies.
May improve accuracy of automated colony pickers. |
| Complete with LB Agar, S-Gal, Kanamycin, IPTG |
No need to make up separate solutions of X-gal, Kanamycin and IPTG, saving time and effort.
No need to wait for media to cool before adding X-gal and IPTG.
No need for toxic solvents, such as DMF. |
| Convenient packaging. Each pouch holds the required amount of S-Gal/Kanamycin/LB Agar Blend powder to make up to 500 ml of media. |
Ease of use for the researcher. Just add pre-blended powder to 500 ml of water, autoclave, and pour plates.
Saves time and labor. |
| S-Gal is not heat labile. |
Can be autoclaved or microwaved.
Provides flexibility to user. |
| S-Gal is not light sensitive. |
No need to shield product or plates from light.
Consistent performance and reliable results. |
Just add water to S-Gal/Kanamycin/LB Agar Blend, autoclave and pour your plates.
It's that easy!
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S-Gal/LB Agar Blend, without IPTG
Product. No. S 9938 is a complete powder mixture useful for detecting prokaryotic cells which
have been transformed with vectors containing the lacZ gene. It contains S-Gal and LB Agar.
This product does not contain IPTG and is designed for those applications where IPTG is not
required. It also allows the researcher to precisely control the amount of inducer in applications
where this is critical.
| FEATURES |
BENEFITS |
| Faster colony color development. |
Pick colonies for faster results. |
| Lac+
colonies turn an intense black color. |
Easier to distinguish between lac + and lac - colonies.
May improve accuracy of automated colony pickers. |
| Complete with LB Agar and S-Gal |
No need to make up a separate solution of X-gal, saving time and effort.
No need to wait for media to cool before adding X-gal.
No need for toxic solvents, such as DMF. |
| Convenient packaging. Each pouch holds the required amount of S-Gal/LB Agar Blend powder to make up to 500 ml of media. |
Ease of use for the researcher. Just add pre-blended powder to 500 ml of water, autoclave, add antibiotic and/or inducer if desired, and pour plates.
Saves time and labor. |
| S-Gal is not heat labile. |
Can be autoclaved or microwaved.
Provides flexibility to user. |
| S-Gal is not light sensitive. |
No need to shield product or plates from light.
Consistent performance and reliable results. |
Just add water to S-Gal/LB Agar Blend, without IPTG, autoclave and pour your plates.
It's that easy!
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S-Gal/LB Agar Blends and Companion Products
These products are packaged especially for convenience in molecular biology applications.
| Prod. No. |
Description |
Packaging |
| C4478 |
S-Gal/LB Agar Blend complete with IPTG |
Pre-weighed blend of components to make 500 ml of complete agar medium. |
| S1813 |
S-Gal/Kanamycin/LB Agar Blend complete with IPTG |
Pre-weighed blend of components to make 500 ml of complete agar medium. |
| S9938 |
S-Gal/LB Agar Blend, without IPTG |
Pre-weighed blend of components to make 500 ml of complete agar medium. |
| S7313 |
S-Gal, sodium salt |
Water soluble to >50 mg/ml. Available in 100 mg, 1 gram and 5 gram packaging. |
| S9811 |
S-Gal |
Original S-Gal. Available in 100 mg, 1 gram and 5 gram packaging. |
| A2804 |
Ampicillin, sodium salt |
Pre-weighed to give typical working concentrations when added to 1 liter of agar preparation. |
| K0879 |
Kanamycin Sulfate |
Pre-weighed to give typical working concentrations when added to 1 liter of agar preparation. |
| T8032 |
Tetracycline Hydrochloride |
Pre-weighed to give typical working concentrations when added to 1 liter of agar preparation. |
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